通过在非线性氯化铯梯度中进行超速离心纯化禽脑脊髓炎病毒。

Purification of avian encephalomyelitis virus by ultracentrifugation in a nonlinear cesium chloride gradient.

作者信息

Matsumoto M, Wangelin J R, Murphy M L

出版信息

Avian Dis. 1978 Jul-Sep;22(3):496-502.

PMID:212005

Abstract

A rapid and convenient method is described for purifying avian encephalomyelitis virus. Infected embryo homogenate was treated with polyethylene glycol followed by extraction with fluorocarbon. Extracted sample was pelleted at 73,400 x g, resuspended, and subjected to isodensity centrifugation in preformed nonlinear gradient. A distinctive virus band was observed after centrifugation for 120 min at 192,000 x g. The fraction containing the virus band demonstrated the major portion of infectivity and the presence of the virus particles by electron microscopy. The method was reproducible within a limited number of trials.

摘要

本文描述了一种快速便捷的禽脑脊髓炎病毒纯化方法。用聚乙二醇处理感染胚胎匀浆，随后用氟碳化合物进行萃取。萃取后的样品在73,400×g的条件下离心成沉淀，重悬后在预先形成的非线性梯度中进行等密度离心。在192,000×g的条件下离心120分钟后，观察到一条独特的病毒带。含有病毒带的组分显示出大部分的感染性，并且通过电子显微镜观察到病毒颗粒的存在。该方法在有限次数的试验中具有可重复性。