Berger R G
Avian Dis. 1982 Jul-Sep;26(3):534-41.
Chicken embryo brain (CEB) cell cultures support the replication of embryo-adapted strains, vaccine strains, and field isolates of avian encephalomyelitis virus. A centrifugal force of 1,500 X g was applied during virus adsorption. Viral antigen was detected in the infected cells by using the indirect fluorescent-antibody technique (IFAT). Combining the infectivity of the virus in CEB cell culture with the ability to detect viral antigen by the IFAT resulted in the development of a virus-titration method. This in vitro assay proved to be more sensitive than the standard embryo-inoculation assay. It was concluded that the in vitro assay provides a satisfactory alternative to the embryo-inoculation assay.
鸡胚脑细胞（CEB）培养物可支持禽脑脊髓炎病毒的胚胎适应株、疫苗株和野外分离株的复制。在病毒吸附过程中施加1500×g的离心力。通过间接荧光抗体技术（IFAT）在感染细胞中检测病毒抗原。将病毒在CEB细胞培养物中的感染性与通过IFAT检测病毒抗原的能力相结合，从而开发出一种病毒滴定方法。这种体外试验被证明比标准的胚胎接种试验更敏感。得出的结论是，体外试验为胚胎接种试验提供了一种令人满意的替代方法。
