Sato M, Bode H R, Sawada Y
Department of Developmental and Cell Biology, University of California, Irvine 92717.
Dev Biol. 1990 Oct;141(2):412-20. doi: 10.1016/0012-1606(90)90395-y.
The monoclonal antibody, TS19, (Heimfeld et al., 1985), labels the apical surface of ectodermal epithelial cells of tentacles and lower peduncles in Hydra. To investigate the patterning process in a tissue whose original pattern was completely destroyed, the TS19 staining pattern was examined in developing aggregates of Hydra cells. Two types of aggregates were prepared. G-aggregates were made from tissue of the gastric portion of animals and RG-aggregates from gastric tissue allowed to regenerate for 24 hr before making aggregates. G-aggregates were initially TS19-negative, and later dim and uniformly TS19-positive. Thereafter, TS19 staining broke up into brightly stained and unstained regions. The brightly staining regions developed into head or foot structures. The TS19 pattern in RG-aggregates developed differently. Since the initial aggregates contained cells of regenerating tips, they started with TS19-positive cells as well as TS19-negative cells. The numbers of brightly staining TS19-positive cells increased with time. Some patches of these cells developed into head or foot structures, while others did not. These results and a simulation using a reaction-diffusion model suggest that the changes in activation levels affected the temporal changes in the pattern of TS19 staining, and that the de novo pattern formation in hydra can be explained in terms of a process involving activation and inhibition properties.
单克隆抗体TS19（Heimfeld等人，1985年）标记水螅触手和下部柄的外胚层上皮细胞的顶端表面。为了研究一个原始模式已被完全破坏的组织中的模式形成过程，在水螅细胞发育的聚集体中检查了TS19染色模式。制备了两种类型的聚集体。G聚集体由动物胃部分的组织制成，RG聚集体由在制备聚集体前已再生24小时的胃组织制成。G聚集体最初是TS19阴性的，后来变暗淡并均匀地呈TS19阳性。此后，TS19染色分解为染色明亮和未染色的区域。染色明亮的区域发育成头部或足部结构。RG聚集体中的TS19模式发育不同。由于最初的聚集体包含再生顶端的细胞，它们一开始既有TS19阳性细胞也有TS19阴性细胞。染色明亮的TS19阳性细胞数量随时间增加。这些细胞的一些斑块发育成头部或足部结构，而其他的则没有。这些结果以及使用反应扩散模型的模拟表明，激活水平的变化影响了TS19染色模式的时间变化，并且水螅中的从头模式形成可以用一个涉及激活和抑制特性的过程来解释。
