Javois L C, Wood R D, Bode H R
Dev Biol. 1986 Oct;117(2):607-18. doi: 10.1016/0012-1606(86)90329-5.
A monoclonal antibody, CP8, has been isolated which displays a position-specific binding pattern to epithelial cells of Hydra oligactis. Antibody binding is restricted to the head of adult animals. When a new head develops during the budding process, CP8 binding is present in the area which will form the head well before morphological signs of it. Similarly, following decapitation as a new head regenerates, CP8 label appears covering a domed area at the apical end of the regenerate before tentacles evaginate delineating the head. When bud development or regeneration is complete, CP8 label is restricted to the new head. Experiments indicate the appearance of CP8 label during the formation of a head correlates closely with the patterning events which result in the determination of the tissue to form a head. The usefulness of CP8 as a diagnostic tool for exploring the dynamics of head pattern formation in hydra is discussed.
一种单克隆抗体CP8已被分离出来，它对寡枝螅的上皮细胞表现出位置特异性结合模式。抗体结合仅限于成年动物的头部。在出芽过程中形成新头部时，CP8结合在形态学迹象出现之前就已出现在将形成头部的区域。同样，在断头后新头部再生时，CP8标记在触手外翻勾勒出头部之前就出现在再生顶端的圆顶区域。当芽发育或再生完成时，CP8标记仅限于新头部。实验表明，在头部形成过程中CP8标记的出现与导致头部组织确定的模式形成事件密切相关。本文讨论了CP8作为探索水螅头部模式形成动态的诊断工具的实用性。
