Department of Pathobiology, College of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
J Physiol Biochem. 2011 Jun;67(2):217-23. doi: 10.1007/s13105-010-0066-y. Epub 2011 Jan 4.
This study was designed to examine the effects of intracerebroventricular injection of DL-AP5 (N-methyl-D-aspartate (NMDA) receptor antagonist) and glutamate on ghrelin-induced feeding behavior in 3-h food-deprived (FD3) broiler cockerels. At first, guide cannula was surgically implanted in the right lateral ventricle of chickens. In experiment 1, birds were intracerebroventricularly injected with 0, 2.5, 5, and 10 nmol of DL-AP5. In experiment 2, chickens received 5 nmol DL-AP5 prior to the injection of 0.6 nmol ghrelin. In experiment 3, birds were administered with 0.6 nmol ghrelin after 300 nmol glutamate, and the cumulative feed intake was determined at 3-h postinjection. The results of this study showed that the intracerebroventricular injection of DL-AP5 increased food consumption in FD3 broiler cockerels (P ≤ 0.05), and this increase occurs in a dose-dependent manner. Moreover, the decreased food intake induced with the intracerebroventricular injection of ghrelin was additively enhanced by pretreatment with glutamate, and this effect was attenuated by DL-AP5 administration(P ≤ 0.05).These results suggest that there is an interaction between ghrelin and glutamatergic system (through NMDA receptor) on food intake in broiler cockerels.
本研究旨在探讨侧脑室注射 DL-AP5（N-甲基-D-天冬氨酸（NMDA）受体拮抗剂）和谷氨酸对 3 小时禁食（FD3）肉鸡促胃液素诱导摄食行为的影响。首先，通过手术将引导套管植入鸡的右侧侧脑室。在实验 1 中，鸡被侧脑室注射 0、2.5、5 和 10 nmol 的 DL-AP5。在实验 2 中，鸡在注射 0.6 nmol 促胃液素前接受 5 nmol DL-AP5。在实验 3 中，鸡在 300 nmol 谷氨酸后给予 0.6 nmol 促胃液素，在注射后 3 小时测定累积采食量。本研究结果表明，侧脑室注射 DL-AP5 增加了 FD3 肉鸡的食物摄入量（P≤0.05），且这种增加呈剂量依赖性。此外，用谷氨酸预处理可增强促胃液素诱导的摄食减少的相加作用，而这种作用可被 DL-AP5 给药减弱（P≤0.05）。这些结果表明，在肉鸡中，促胃液素和谷氨酸能系统（通过 NMDA 受体）之间存在相互作用，从而影响摄食。
