Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453, Tehran, Iran.
Arch Razi Inst. 2021 Sep 1;76(3):537-551. doi: 10.22092/ari.2020.351260.1513. eCollection 2021 Summer.
This study purposed to discover the connection between the central glutamatergic and histaminergic systems on feeding behavior in layer chickens. In the first experiment, chicks obtained intracerebroventricular (ICV) injections of saline (control solution), α-FMH (250 nmol), glutamate (300 nmol), and α-FMH + glutamate. Experiments 2-6 were comparable to the first experiment, apart from the birds being injected with chlorpheniramine (histamine H receptor antagonist, 300 nmol), famotidine (histamine H receptor antagonist, 82 nmol), and thioperamide (histamine H3 receptor antagonist, 300 nmol) instead of α-FMH. In Experiment five, experimental groups were divided into (A) control solution, (B) MK-801 (N-methyl-D-aspartate receptor antagonist, 15 nmol), (C) histamine (300 nmol) and (D) MK-801 + histamine. Experiments 6-10 and Experiment five were similar apart from the ICV injections of CNQX (AMPA receptor antagonist, 360 nm), UBP-302 (Kainate receptor antagonist, 390 nm), AIDA (mGluR antagonist, 2 nmol), LY341495 (mGluR antagonist, 150 nmol), and UBP1112 (mGluR antagonist, 2 nmol) given instead of MK-801. Afterward, cumulative food intake was recorded at30, 60, and 120 minutes after the injection process. According to the results, ICV injection of glutamate considerably reduced food intake (<0.05). Co-injection of α-FMH + glutamate and/or chlorpheniramine + glutamate reduced the hypophagic influence of glutamate (<0.05), whereas thioperamide + glutamate augmented glutamate-induced hypophagia in neonatal chicks (<0.05). Co-injection of MK-801 + histamine or UBP-302 + histamine reduced the hypophagic influence of the histamine (<0.05), whereas LY341495 + histamine augmented the hypophagic influence of the histamine (<0.05). Given the results, it is suggested that the effect of the connection between these systems on the process of food intake regulation is mediated by H and H histamines as well as NMDA, Kainate, and mGluR glutamate receptors in neonatal layer chickens.
本研究旨在探索禽类中枢谷氨酸能和组胺能系统在摄食行为中的关系。在第一个实验中，小鸡接受了脑室内（ICV）注射生理盐水（对照溶液）、α-FMH（250nmol）、谷氨酸（300nmol）和α-FMH+谷氨酸。实验 2-6 与第一个实验类似，只是小鸡接受了氯苯那敏（组胺 H 受体拮抗剂，300nmol）、法莫替丁（组胺 H 受体拮抗剂，82nmol）和噻哌酰胺（组胺 H3 受体拮抗剂，300nmol）而非α-FMH 的注射。在实验 5 中，实验组分为（A）对照溶液、（B）MK-801（N-甲基-D-天冬氨酸受体拮抗剂，15nmol）、（C）组胺（300nmol）和（D）MK-801+组胺。实验 6-10 和实验 5 类似，只是小鸡接受了 CNQX（AMPA 受体拮抗剂，360nm）、UBP-302（KA 受体拮抗剂，390nm）、AIDA（mGluR 拮抗剂，2nmol）、LY341495（mGluR 拮抗剂，150nmol）和 UBP1112（mGluR 拮抗剂，2nmol）而非 MK-801 的脑室内注射。之后，在注射后 30、60 和 120 分钟记录累积食物摄入量。结果显示，脑室内注射谷氨酸显著减少了食物摄入量（<0.05）。共注射α-FMH+谷氨酸和/或氯苯那敏+谷氨酸可降低谷氨酸的致厌食作用（<0.05），而噻哌酰胺+谷氨酸则增强了新生小鸡中谷氨酸引起的厌食（<0.05）。共注射 MK-801+组胺或 UBP-302+组胺可降低组胺的致厌食作用（<0.05），而 LY341495+组胺则增强了组胺的致厌食作用（<0.05）。因此，我们推测这些系统之间的联系对禽类食物摄入调节过程的影响是通过 NMDA、KA 和 mGluR 谷氨酸受体以及 H1 和 H3 组胺受体介导的。
