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天蓝色链霉菌漆酶在用六氰合铁酸钾浸泡后的结构，分辨率提高至2.3 Å。

Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å.

作者信息

Skálová Tereza, Dušková Jarmila, Hašek Jindřich, Stěpánková Andrea, Koval Tomáš, Østergaard Lars Henrik, Dohnálek Jan

机构信息

Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovského nám. 2, 162 06 Praha 6, Czech Republic.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):27-32. doi: 10.1107/S1744309110046099. Epub 2010 Dec 21.

DOI:10.1107/S1744309110046099
PMID:21206017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3079965/
Abstract

The paper reports the structure of the small laccase from Streptomyces coelicolor determined from a crystal soaked with potassium hexacyanoferrate [K4Fe(CN)6]. The decolorization of the natively blue crystal observed upon soaking indicates the reduction of the enzyme in the crystal. The ligand binds between laccase molecules and stabilizes the crystal. The increased diffraction limit of the diffraction data collected from this crystal enabled the refinement of the small laccase structure at 2.3 Å resolution, which is the highest resolution obtained to date.

摘要

该论文报道了通过用铁氰化钾[K4Fe(CN)6]浸泡的晶体确定的天蓝色链霉菌小漆酶的结构。浸泡后观察到天然蓝色晶体的脱色表明晶体中酶的还原。配体在漆酶分子之间结合并稳定晶体。从该晶体收集的衍射数据的衍射极限提高，使得能够在2.3 Å分辨率下精修小漆酶结构，这是迄今为止获得的最高分辨率。

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Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å.天蓝色链霉菌漆酶在用六氰合铁酸钾浸泡后的结构，分辨率提高至2.3 Å。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):27-32. doi: 10.1107/S1744309110046099. Epub 2010 Dec 21.
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本文引用的文献

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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
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Structure-function studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds.对一种白黑脉菌漆酶的结构-功能研究揭示了酚类化合物的氧化途径。
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X-ray structure of a two-domain type laccase: a missing link in the evolution of multi-copper proteins.双结构域I型漆酶的X射线结构：多铜蛋白进化中的缺失环节。
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The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases.来自天蓝色链霉菌的小型漆酶结构揭示了漆酶与亚硝酸还原酶之间的联系。
J Mol Biol. 2009 Jan 30;385(4):1165-78. doi: 10.1016/j.jmb.2008.11.024. Epub 2008 Nov 25.
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Crystallization and preliminary X-ray diffraction analysis of the small laccase from Streptomyces coelicolor.天蓝色链霉菌小漆酶的结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt 12):1077-9. doi: 10.1107/S1744309107060721. Epub 2007 Nov 30.