Skálová Tereza, Dohnálek Jan, Ostergaard Lars Henrik, Ostergaard Peter Rahbek, Kolenko Petr, Dusková Jarmila, Hasek Jindrich
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského nám. 2, 162 06 Praha 6, Czech Republic.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt 12):1077-9. doi: 10.1107/S1744309107060721. Epub 2007 Nov 30.
The small bacterial laccase from the actinobacterium Streptomyces coelicolor which lacks the second of the three domains of the laccases structurally characterized to date was crystallized. This multi-copper phenol oxidase crystallizes in a primitive tetragonal lattice, with unit-cell parameters a = b = 179.8, c = 175.3 A. The crystals belong to either space group P4(1)2(1)2 or P4(3)2(1)2. The self-rotation function shows the presence of a noncrystallographic threefold axis in the structure. Phases will be determined from the anomalous signal of the natively present copper ions.
来自天蓝色链霉菌的小型细菌漆酶被结晶出来，该漆酶缺乏迄今为止在结构上已表征的漆酶三个结构域中的第二个结构域。这种多铜酚氧化酶以原始四方晶格结晶，晶胞参数为a = b = 179.8，c = 175.3 Å。晶体属于空间群P4(1)2(1)2或P4(3)2(1)2。自旋转函数表明该结构中存在一个非晶体学三重轴。将根据天然存在的铜离子的反常信号确定相位。
