Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, 2800 Kgs. Lyngby, Denmark.
Biomed Microdevices. 2011 Apr;13(2):383-92. doi: 10.1007/s10544-010-9507-1.
We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.
我们提出了一种聚合物和不锈钢的混合芯片，用于高通量连续电穿孔悬浮细胞。该芯片由两个平行的不锈钢网格电极构成，其方向垂直于液体流动。与主通道相比，网格中的微米大小的孔的相对较高的流体动力阻力迫使网格之间的流速几乎均匀。从而可以实现细胞的非常均匀的电穿孔。已成功地用 mRNA 对 2000 万个人类树突状细胞进行了电穿孔。该芯片的性能与传统的电穿孔管相似，但没有要电穿孔的细胞数量的上限。该器件由两个女性鲁尔部件构成，并且可以很容易地与其他微流控组件集成。此外，它由注塑成型的聚合物部件和市售的不锈钢网制成，因此适合于廉价的批量生产。
