Britz-McKibbin Philip
Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, Canada.
Methods Mol Biol. 2011;708:229-46. doi: 10.1007/978-1-61737-985-7_14.
Metabolomics is a rapidly emerging field of functional genomics research whose aim is the comprehensive analysis of low molecular weight metabolites in a biological sample. Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) represents a promising hyphenated microseparation platform in metabolomics, since a majority of primary metabolites are intrinsically polar. CE-ESI-MS offers a convenient format for the separation of complex mixtures of cationic, anionic, and/or zwitterionic metabolites, as well as their isobaric/isomeric ions without complicated sample handling. Moreover, online sample preconcentration with desalting is readily integrated during separation prior to ionization, where the migration behavior and ionization response of metabolites can be predicted based on their fundamental physicochemical properties. Herein, we describe recent developments in CE-ESI-MS with emphasis on practical protocols necessary for realizing reliable analyses as applied to targeted metabolite profiling and untargeted metabolomic studies in various biological samples.
代谢组学是功能基因组学研究中一个迅速兴起的领域，其目的是对生物样品中的低分子量代谢物进行全面分析。毛细管电泳-电喷雾电离-质谱联用（CE-ESI-MS）是代谢组学中一个很有前景的联用微分离平台，因为大多数初级代谢物本质上是极性的。CE-ESI-MS为分离阳离子、阴离子和/或两性离子代谢物的复杂混合物及其等压/同分异构离子提供了一种便捷的方式，无需复杂的样品处理。此外，在电离前的分离过程中很容易集成在线脱盐样品预浓缩，在此过程中可以根据代谢物的基本物理化学性质预测其迁移行为和电离响应。在此，我们描述了CE-ESI-MS的最新进展，重点是在各种生物样品中进行靶向代谢物分析和非靶向代谢组学研究时实现可靠分析所需的实用方案。
