Liu Jian-ren, Song Shui-jiang, Wei Er-qing, Wang Men-ling, Ge Qiu-fu, Li Wei, Liu Ruo-ying
Department of Neurology, 2nd Affiliated Hospital, College of Medical Sciences, Zhejiang Univercity, Hangzhou 310009, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 May;19(2):155-9.
To investigate the protective effect of monosialoganglioside (GM1) on injury induced by oxygen glucose deprivation/reperfusion (OGD/Rep) in rat hippocampal slices.
The protective effects of GM1 on hippocampal slices after OGD/Rep were observed by detecting the light transmittance (LT) changes of rat hippocampal slices and 2, 3, 5-triphenyltetrazolium chloride (TTC) staining of rat hippocampal slices.
(1) In four groups treated with 0 (control), 0.1, 1.0, 10 micromol/L GM1, the peak of light transmittance (LT) in the slices treated with 1.0 micromol/L GM1 was significantly lower than that of the control and the group treated with 0.10 micromol/L GM1 (P < 0.01, ANOVA), while the peak of LT in the slices treated with 10.0 micromol/L GM1 was significantly lower than that of the other groups (P < 0.01, ANOVA). The time to reach the peak of LT in four groups was significantly different from each other (P < 0.05, Kruskal-Wallis test). The time to reach the peak of LT in the group treated with 1 micromol/L GM1 was the significantly longer than that in the control (P < 0.01, Mann-Whitney U test). (2) There was characteristic dose-response relationship between GM1 and TTC staining of rat hippocampal slices. In the five groups, treated with 0 (control), 0.01, 0.1, 1.0, 10 micromol/L GM1 respectively, TTC staining in the group treated with 1 micromol/L GM1 was the deepest (P < 0.05 vs. control, 0.01 and 0.1 micromol/L GM1 group, ANOVA), and the next was in the group treated with 10 micromol/L GM1 (P < 0.05 vs. control and 0.01 micromol/L GM1 group, ANOVA).
GM1 could protect injury induced by OGD/Rep in rat hippocampal slices effectively in vitro.
探讨单唾液酸神经节苷脂（GM1）对大鼠海马脑片氧糖剥夺/复灌注（OGD/Rep）损伤的保护作用。
通过检测大鼠海马脑片的透光率（LT）变化及对大鼠海马脑片进行2,3,5-三苯基四氮唑氯化物（TTC）染色，观察GM1对OGD/Rep后海马脑片的保护作用。
（1）在分别用0（对照）、0.1、1.0、10 μmol/L GM1处理的四组中，用1.0 μmol/L GM1处理的脑片的透光率（LT）峰值显著低于对照组和用0.10 μmol/L GM1处理的组（P<0.01，方差分析），而用10.0 μmol/L GM1处理的脑片的LT峰值显著低于其他组（P<0.01，方差分析）。四组达到LT峰值的时间彼此显著不同（P<*0.05，Kruskal-Wallis检验）。用1 μmol/L GM1处理的组达到LT峰值的时间显著长于对照组（P<0.01，Mann-Whitney U检验）。（2）GM1与大鼠海马脑片的TTC染色之间存在特征性剂量反应关系。在分别用0（对照）、0.01、0.1、1.0、10 μmol/L GM1处理的五组中，用1 μmol/L GM1处理的组的TTC染色最深（与对照组、0.01和0.1 μmol/L GM1组相比，P<0.05，方差分析），其次是用10 μmol/L GM1处理的组（与对照组和0.01 μmol/L GM1组相比，P<0.05，方差分析）。
GM1在体外可有效保护大鼠海马脑片免受OGD/Rep损伤。
