Yu Jun, Zhu Miao-Zhang, Cheng Bao-Ying, Lu Shun-Yan, Dong Ming-Qing
Department of Physiology, Fourth Military Medical University, Xi'an 710032, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 Feb;19(1):8-11.
To investigate how vasonatrin peptide (VNP) can attenuate the growth-promoting effect of hypoxia in cardiac fibroblasts cultured from neonatal rats.
The cultured cardiac fibroblasts were divided randomly into four groups: control group, hypoxia group, hypoxia + VNP group and hypoxia + 8-Bromo-cGMP group. The growth of cardiac myocytes was measured by the means of MTT method. The effect of VNP on the intracellular level of cGMP and PCNA were measured by the means of radioimmunoassay and immunohistochemistry stain respectively.
Hypoxia (24 h) significantly increased the MTT A490nm value of cardiac fibroblasts (P < 0.05 vs control group). Both VNP (10(-7) mol/L) and 8-Bromo-cGMP (10(-3) mol/L) decreased MTT A490 nm value in cardiac fibroblast (P < 0.05 vs hypoxia group). VNP (10(-7) mol/L) increased the intracellular level of cGMP (P < 0.05 vs control and hypoxia group). Hypoxia (24 h) significantly increased the expression of proliferating cell nuclear antigen (PCNA) in cardiac myocytes (P < 0.05, vs control group), but VNP (10(-7) mol/L) decreased it.
VNP can attenuate hypoxia-induced growth-promoting effect in cardiac fibroblasts which is associated with the changes of cGMP and PCNA.
研究血管钠肽（VNP）如何减弱新生大鼠培养的心脏成纤维细胞中缺氧的促生长作用。
将培养的心脏成纤维细胞随机分为四组：对照组、缺氧组、缺氧+VNP组和缺氧+8-溴-cGMP组。采用MTT法检测心肌细胞的生长情况。分别采用放射免疫分析法和免疫组织化学染色法检测VNP对细胞内cGMP水平和增殖细胞核抗原（PCNA）的影响。
缺氧（24小时）显著增加了心脏成纤维细胞的MTT A490nm值（与对照组相比，P<0.05）。VNP（10⁻⁷mol/L）和8-溴-cGMP（10⁻³mol/L）均降低了心脏成纤维细胞的MTT A490nm值（与缺氧组相比，P<0.05）。VNP（10⁻⁷mol/L）增加了细胞内cGMP水平（与对照组和缺氧组相比，P<0.05）。缺氧（24小时）显著增加了心肌细胞中增殖细胞核抗原（PCNA）的表达（与对照组相比，P<0.05），但VNP（10⁻⁷mol/L）降低了其表达。
VNP可减弱缺氧诱导的心脏成纤维细胞的促生长作用，这与cGMP和PCNA的变化有关。
