Guo H T, Zhu M Z, Lu S Y, Yu J, Dong M Q, Gao Z, Shi P T
Department of Physioloy, Fourth Military Medical University, Xi an 710032.
Sheng Li Xue Bao. 2001 Aug;53(4):286-90.
The purpose of this work was to test the hypothesis that vasonatrin peptide (VNP) can attenuate the growth-promoting effect by hypoxia in cardiac fibroblasts of cultured neonatal rats. Cultured fibroblasts were divided into four groups: control group, hypoxia group, VNP group and VNP+hypoxia group. The growth of cardiac fibroblasts was observed using MTT method and the incorporation of (3)H-TdR, and the effect of VNP on the intracellular level of Ca(2+) was measured by means of interactive laser cytometry. It was found that hypoxia (2% - 3%) increased significantly the MTT optical density (OD) of cardiac fibroblasts (P<0.05 vs control group), but the increase was greatly attenuated in the VNP (10(- 6)mol/L) group and also the incorporation of (3)H-TdR in cardiac fibroblasts (P<0.05 vs hypoxia group). VNP (10(- 6)mol/L) also decreased the intracellular level of Ca(2+) which was increased by hypoxia (P<0.05) as compared with control and hypoxia group. These findings demonstrate that VNP can attenuate the hypoxia-induced growth-promoting effect in cardiac fibroblasts, which may be associated with the elevation of intracellular Ca(2+).
本研究旨在验证血管钠肽（VNP）可减弱缺氧对新生大鼠培养心肌成纤维细胞促生长作用的假说。将培养的成纤维细胞分为四组：对照组、缺氧组、VNP组和VNP +缺氧组。采用MTT法和（3）H-TdR掺入法观察心肌成纤维细胞的生长情况，并用激光共聚焦显微镜检测VNP对细胞内Ca（2+）水平的影响。结果发现，缺氧（2% - 3%）显著增加了心肌成纤维细胞MTT光密度（OD）（与对照组相比，P<0.05），但在VNP（10（-6）mol/L）组中这种增加明显减弱，心肌成纤维细胞中（3）H-TdR的掺入也减少（与缺氧组相比，P<0.05）。VNP（10（-6）mol/L）还降低了缺氧诱导升高的细胞内Ca（2+）水平（与对照组和缺氧组相比，P<0.05）。这些结果表明，VNP可减弱缺氧诱导的心肌成纤维细胞促生长作用，这可能与细胞内Ca（2+）升高有关。
