髁突软骨和关节窝的组织化学研究在下颌前伸过程中。
A histochemical study on condylar cartilage and glenoid fossa during mandibular advancement.
机构信息
Department of Orthodontics, University of Sydney, Sydney Dental Hospital, NSW Australia.
出版信息
Angle Orthod. 2011 Mar;81(2):270-6. doi: 10.2319/021710-99.1.
OBJECTIVE
To evaluate cellular hypertrophic activities in the mandibular condylar cartilage (MCC) and the glenoid fossa (GF) during mandibular advancement in the temporomandibular joint (TMJ) of Sprague-Dawley rats, as evidenced by fibroblast growth factor 8 (FGF8).
METHODS AND MATERIALS
Fifty-five female 24-day-old Sprague-Dawley rats were randomly divided into four experimental and control groups, with a mandibular advancement appliance on the experimental rats' lower incisors. The rats were euthanized on days 3, 14, 21, and 30 of the study, and their TMJ was prepared for a immunohistochemical staining procedure to detect FGF8.
RESULTS
FGF8 expression was significantly higher among the experimental rats (P = .002). Patterns of ascension and descension of FGF8 expression were similar in experimental and control samples. The results show an overall enhanced osteogenic transition occurring in both the MCC and the GF in experimental rats in comparison with controls. The level of cellular changes in the MCC is remarkably higher than in the GF.
CONCLUSION
In the MCC and the GF, cellular morphologic and hypertrophic differentiations increase significantly during mandibular advancement. It is also concluded that endochondral ossification in the MCC and intramembranous ossification in the GF occur during adaptive remodeling.
目的
通过纤维母细胞生长因子 8（FGF8）评估髁突软骨（MCC）和关节窝（GF）在 TMJ 中下颌前伸时的细胞肥大活性。
方法和材料
55 只 24 天大的雌性 Sprague-Dawley 大鼠随机分为四组实验组和对照组，实验组大鼠的下切牙上带有下颌前伸矫治器。研究第 3、14、21 和 30 天处死大鼠，制备 TMJ 进行免疫组化染色以检测 FGF8。
结果
实验组大鼠的 FGF8 表达明显更高（P = .002）。实验组和对照组样本的 FGF8 表达上升和下降模式相似。结果表明，与对照组相比，实验组大鼠的 MCC 和 GF 中均发生了整体增强的成骨转化。MCC 中的细胞变化水平明显高于 GF。
结论
在 MCC 和 GF 中，下颌前伸过程中细胞形态和肥大分化显著增加。此外，还得出结论，MCC 中的软骨内骨化和 GF 中的膜内骨化在适应性重塑过程中发生。
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