Palatroni P, Gabrielli M G, Taborro R
Department of Cellular Biology, University of Camerino, Italy.
Acta Histochem. 1990;88(2):187-98. doi: 10.1016/S0065-1281(11)80132-7.
Carbonic anhydrase activity has been studied during retina development in 2 mammalian species, guinea pig and rat, which differ for birth time and gestational period as being precocial and altricial respectively. For both species, the definitive pattern of enzyme distribution corresponds to the localization of the reaction product in the Müller glial cells at the level of nucleus, perikaryon, lateral processes, and end-feet. Only in the rat retina, staining has been observed also in some amacrine cells. The results of either in situ or extra situm investigations showed that, according to tissue maturity, in the precocial species, the definitive expression of carbonic anhydrase is reached at birth time. In the altricial species, on the contrary, maturity is very delayed and may be recognized at only the 12th d of postnatal life. Present findings confirm that carbonic anhydrase is a marker for the maturity of the retinal glial cells.
在两种哺乳动物（豚鼠和大鼠）的视网膜发育过程中对碳酸酐酶活性进行了研究，这两种动物的出生时间和妊娠期不同，分别为早熟型和晚熟型。对于这两个物种，酶分布的最终模式对应于反应产物在穆勒神经胶质细胞的细胞核、核周体、外侧突起和终足水平的定位。仅在大鼠视网膜中，在一些无长突细胞中也观察到了染色。原位或体外研究的结果表明，根据组织成熟度，在早熟物种中，碳酸酐酶在出生时达到最终表达。相反，在晚熟物种中，成熟非常延迟，可能仅在出生后第12天才可识别。目前的研究结果证实碳酸酐酶是视网膜神经胶质细胞成熟的标志物。
