School of Environmental Studies, Jadavpur University, Kolkata, 700 032, India.
Appl Microbiol Biotechnol. 2011 Apr;90(1):321-30. doi: 10.1007/s00253-010-3041-1. Epub 2011 Jan 6.
A polymethylmethacrylate (PMMA) conico-cylindrical flask (CCF) with an inner arrangement consisting of eight equidistantly spaced rectangular strips mounted radially on a circular disk to provide additional surface area for microbial attachment and ports to allow air supply was employed for melanin production by Shewanella colwelliana and antibiotic production by Pseudoalteromonas rubra. The design allowed comparison of production between (1) CCF with hydrophobic surface (PMMA-CCF), (2) CCF with hydrophilic glass surface (GS-CCF), and (3) standard unbaffled Erlenmeyer flask (EF). Melanin production in the PMMA-CCF was higher by at most 33.5% and growth of S. colwelliana by at most 309.2% compared to the other vessels. Melanin synthesis was positively correlated with reactor surface area and hydrophobicity, suspended cell growth, and biofilm formation. Antibiotic production in the EF was higher by at most 83.3%, but growth of P. rubra was higher in the PMMA-CCF by at most 54.5% compared to the other vessels. A hydrophilic vessel surface, abundant air supply, but low shear stress enhanced antibiotic production. The CCF together with the EF allowed identification of the crucial parameters (vessel surface characteristics, growth, biofilm formation, and aeration) influencing productivity, knowledge of which in the initial stages of process development will facilitate informed decisions at the later phases.
采用一种内部排列有八个等距间隔的矩形条的聚甲基丙烯酸甲酯（PMMA）锥形-圆柱形瓶（CCF），这些矩形条径向安装在一个圆形磁盘上，以提供更多的微生物附着表面，并设置端口以允许空气供应，用于希瓦氏菌（Shewanella colwelliana）生产黑色素和红色假交替单胞菌（Pseudoalteromonas rubra）生产抗生素。该设计允许比较（1）具有疏水性表面的 CCF（PMMA-CCF）、（2）具有亲水性玻璃表面的 CCF（GS-CCF）和（3）标准无挡板锥形瓶（EF）之间的生产情况。与其他容器相比，PMMA-CCF 中的黑色素产量最高增加了 33.5%，希瓦氏菌的生长最高增加了 309.2%。黑色素合成与反应器表面积和疏水性、悬浮细胞生长和生物膜形成呈正相关。EF 中的抗生素产量最高增加了 83.3%，但与其他容器相比，PMMA-CCF 中的红色假交替单胞菌生长最高增加了 54.5%。亲水容器表面、充足的空气供应但低剪切应力增强了抗生素的生产。CCF 与 EF 一起，可以确定影响生产力的关键参数（容器表面特性、生长、生物膜形成和曝气），在工艺开发的早期阶段了解这些参数将有助于在后期阶段做出明智的决策。
