a School of Environmental Studies , Jadavpur University , Kolkata , India.
Biofouling. 2015;31(3):283-96. doi: 10.1080/08927014.2015.1038705.
A direct relationship between biofilm formation and melanogenesis in Shewanella colwelliana with increased oyster recruitment is already established. Previously, S. colwelliana was grown in a newly patented biofilm-cultivation device, the conico-cylindrical flask (CCF), offering interchangeable hydrophobic/hydrophilic surfaces. Melanization was enhanced when S. colwelliana was cultivated in a hydrophobic vessel compared with a hydrophilic vessel. In the present study, melanogenesis in the CCF was positively correlated with increased architectural parameters of the biofilm (mean thickness and biovolume obtained by confocal laser scanning microscopy) and melanin gene (melA) expression observed by densitometry. Niche intertidal conditions were mimicked in a process operated in an ultra-low-speed rotating disk bioreactor, which demonstrated enhanced biofilm formation, melanogenesis, exopolysaccharide synthesis and melA gene expression compared with a process where 12-h periodic immersion and emersion was prevented. The wettability properties of the settling plane as well as intermittent wetting and drying, which influenced biofilm formation and melA expression, may affect oyster settlement in nature.
已经证实希瓦氏菌属（Shewanella colwelliana）的生物膜形成与黑色素生成之间存在直接关系，且这种关系与牡蛎的附着增加有关。先前，希瓦氏菌属（Shewanella colwelliana）在一种新获得专利的生物膜培养装置——锥形-圆柱形瓶（CCF）中进行培养，该装置提供了可互换的疏水/亲水表面。与亲水容器相比，当在疏水容器中培养希瓦氏菌属（Shewanella colwelliana）时，其黑色素生成得到了增强。在本研究中，CCF 中的黑色素生成与生物膜的结构参数（通过共聚焦激光扫描显微镜获得的平均厚度和生物体积）的增加以及黑色素基因（melA）的表达呈正相关，通过密度计观察到。在超低转速旋转盘生物反应器中进行的过程中模拟了生态位潮间带条件，与阻止 12 小时周期性浸入和露出的过程相比，该过程显示出增强的生物膜形成、黑色素生成、胞外多糖合成和 melA 基因表达。沉降平面的润湿性特性以及间歇性的润湿和干燥会影响生物膜的形成和 melA 表达，这可能会影响自然界中牡蛎的附着。
