Liu Pei-sheng, Chen Xin, Liu Ming
Department of Thoracic and Cardiovascular Surgery, Nanjing Heart Disease Hospital, Nanjing Medical University, Nanjing 210006, China.
Zhonghua Wai Ke Za Zhi. 2010 Dec 15;48(24):1881-4.
To analyze the transmural lesions of different parts of the pig heart atrium received different times of ablation applied with Atricure bipolar radiofrequency system.
Six fresh (ex vivo time<20 min) pig hearts with atrium preserved intact were used as the experimental objects and experimental groups were divided according to the ablation position. The Atricure bipolar radiofrequency system was applied in the ablation of the parts of the atrium, such as posterior wall of left atrium, anterior wall of left atrium, anterior wall of right atrium and posterior wall of left atrium close to mitral posterior ring. Ablate the position of the atrium lengthened about 2.0 cm with the same thickness with an interval of 0.5 cm for 4 times respectively, also recording the time of every ablation. For each part and each time of ablation, the ablated atrial tissue was preserved with 4% formaldehyde and 5% glutaraldehyde, and was sent for observation under light microscope and transmission electron microscope. The ablation time and lesion were analyzed statistically.
In the same position of the atrium, ablation time decreased with the times of the ablation, in different position of the atrium with same time of ablation, time showed a positive proportion with the thickness of the atrium.
Atricure bipolar radiofrequency system is very safe and efficient, also convenient for manipulation. With regard to the relatively thinner part of the atrium, such as posterior wall and anterior wall of left atrium, at least two times of ablation can ensure transmural lesion of the atrial tissue, but to the position of the atrium such as anterior wall of right atrium and posterior wall of left atrium close to mitral posterior ring, 3 to 4 times of ablation can ensure transmural lesion of the atrial tissue.
分析应用Atricure双极射频系统在不同时间对猪心脏心房不同部位进行消融后的透壁损伤情况。
选取6个心房完整的新鲜猪心脏（离体时间<20分钟）作为实验对象，根据消融部位进行分组。应用Atricure双极射频系统对左心房后壁、左心房前壁、右心房前壁及左心房后壁靠近二尖瓣后环等心房部位进行消融。在心房各部位消融长度约2.0 cm、厚度相同、间隔0.5 cm处分别进行4次消融，并记录每次消融时间。对每个部位每次消融后的心房组织用4%甲醛和5%戊二醛固定，送光镜和透射电镜观察。对消融时间和损伤情况进行统计学分析。
在心房同一部位，消融时间随消融次数增加而减少；在心房不同部位，相同消融次数时，消融时间与心房厚度呈正相关。
Atricure双极射频系统安全有效，操作方便。对于心房相对较薄的部位，如左心房后壁和前壁，至少两次消融可确保心房组织透壁损伤；但对于右心房前壁及左心房后壁靠近二尖瓣后环等部位，3至4次消融可确保心房组织透壁损伤。
