Xia Shu-Yue, Kang Jian, Jiang Yan-Duo, Huang De-Sheng, Wang Shi, Pang Bao-Sen
Institution of Respiratory Disease, the First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2010 Nov;33(11):806-10.
to explore the effect of simvastatin on alveolar epithelial cells and the expression of vascular endothelial growth factor (VEGF) in cigarette smoking-induced emphysema in rats.
twenty-four, 12-week-old healthy male and female Wistar rats were randomly divided into 4 groups of 6 each: a control (W) group, a smoking (Sm) group, a simvastatin (St) group, and a smoking-simvastatin (SmSt) group. The rats were simultaneously fed, and kept in individual cages for 16 weeks. The VEGF level in lung tissue and bronchoalveolar lavage fluid (BALF) of each group was measured by ELISA. The expression of VEGF mRNA was determined by RT-PCR. The expressions of VEGF and proliferating cell nuclear antigen (PCNA) were determined by two-step immunohistochemistry assay. One-way ANOVA and LSD-t test were used for statistical analysis.
the percentage of PCNA-positively stained alveolar epithelial cells was significantly higher in the SmSt group [(10.3 ± 2.0)%] than in the Sm group [(4.8 ± 0.8)%]. The levels of VEGF in BALF and lung tissue homogenate of the SmSt group [(187 ± 15) ng/L and (6782 ± 50) ng/L] were similar to the W group [(200 ± 20) ng/L and (7558 ± 330) ng/L], but were significantly higher than that in the Sm group [(71 ± 16) ng/L and (4149 ± 110) ng/L]. VEGF expression in alveolar and bronchial epithelial cells of rats in the SmSt group [(67.7 ± 5.0)% and (49.0 ± 3.0)%], was similar to the W group [(68.3 ± 3.3)% and (51.3 ± 2.9)%]. But the level of VEGF expression was significantly increased as compared to that in the Sm group [(27.0 ± 5.9)% and (16.3 ± 2.7)%]. SmSt group vs Sm group t = 1.117 - 12.001, all P < 0.01.
simvastatin ameliorated the development of cigarette smoke-induced COPD in rats, partly by promoting alveolar epithelial cell proliferation and up-regulating the expression of VEGF.
探讨辛伐他汀对吸烟诱导的大鼠肺气肿模型中肺泡上皮细胞及血管内皮生长因子（VEGF）表达的影响。
将24只12周龄健康Wistar大鼠随机分为4组，每组6只：对照组（W组）、吸烟组（Sm组）、辛伐他汀组（St组）和吸烟 - 辛伐他汀组（SmSt组）。大鼠同时喂养，并单独饲养于笼中16周。采用ELISA法检测各组大鼠肺组织及支气管肺泡灌洗液（BALF）中VEGF水平。采用RT - PCR法检测VEGF mRNA表达。采用两步免疫组化法检测VEGF和增殖细胞核抗原（PCNA）的表达。采用单因素方差分析和LSD - t检验进行统计学分析。
SmSt组PCNA阳性染色的肺泡上皮细胞百分比[（10.3±2.0）%]显著高于Sm组[（4.8±0.8）%]。SmSt组BALF和肺组织匀浆中VEGF水平[（187±15）ng/L和（6782±50）ng/L]与W组[（200±20）ng/L和（7558±330）ng/L]相似，但显著高于Sm组[（71±16）ng/L和（4149±110）ng/L]。SmSt组大鼠肺泡和支气管上皮细胞中VEGF表达[（67.7±5.0）%和（49.0±3.0）%]与W组[（68.3±3.3）%和（51.3±2.9）%]相似，但与Sm组相比VEGF表达水平显著升高[（27.0±5.9）%和（16.3±2.7）%]。SmSt组与Sm组比较，t = 1.117 - 12.001，均P < 0.01。
辛伐他汀可改善吸烟诱导的大鼠慢性阻塞性肺疾病的发展，部分是通过促进肺泡上皮细胞增殖和上调VEGF表达实现的。
