Department of Oral Functional Prosthodontics, Division of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Kita-ku, Sapporo, Hokkaido, Japan.
Nanotechnology. 2011 Feb 11;22(6):065102. doi: 10.1088/0957-4484/22/6/065102. Epub 2011 Jan 7.
A recent study showed that carbon nanohorns (CNHs) have biocompatibility and possible medical uses such as in drug delivery systems. It was reported that some kinds of carbon nanomaterials such as carbon nanotubes were useful for bone formation. However, the effect of CNHs on bone tissue has not been clarified. The purpose of this study was to evaluate the effect of CNHs on bone regeneration and their possible application for guided bone regeneration (GBR). CNHs dispersed in ethanol were fixed on a porous polytetrafluoroethylene membrane by vacuum filtration. Cranial defects were created in rats and covered by a membrane with/without CNHs. At two weeks, bone formation under the membrane with CNHs had progressed more than under that without CNHs and numerous macrophages were observed attached to CNHs. At eight weeks, there was no significant difference in the amount of newly formed bone between the groups and the appearance of macrophages was decreased compared with that at two weeks. Newly formed bone attached to some CNHs directly. These results suggest that macrophages induced by CNHs are related to bone regeneration. In conclusion, the present study indicates that CNHs are compatible with bone tissue and effective as a material for GBR.
最近的一项研究表明，碳纳米角（CNHs）具有生物相容性和潜在的医学用途，例如在药物输送系统中。据报道，某些类型的碳纳米材料，如碳纳米管，对骨形成有用。然而，CNHs 对骨组织的影响尚未阐明。本研究旨在评估 CNHs 对骨再生的影响及其在引导骨再生（GBR）中的潜在应用。通过真空过滤将分散在乙醇中的 CNHs 固定在多孔聚四氟乙烯膜上。在大鼠颅骨上制造缺损，并在有/没有 CNHs 的情况下用膜覆盖。在两周时，在有 CNHs 的膜下的骨形成进展超过了没有 CNHs 的膜下的骨形成，并且观察到许多巨噬细胞附着在 CNHs 上。在八周时，两组之间新形成骨的量没有显著差异，与两周时相比，巨噬细胞的外观减少。新形成的骨直接附着在一些 CNHs 上。这些结果表明，CNHs 诱导的巨噬细胞与骨再生有关。总之，本研究表明 CNHs 与骨组织相容，并且是 GBR 材料的有效选择。
