Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan.
Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan.
Int J Nanomedicine. 2018 Mar 16;13:1643-1652. doi: 10.2147/IJN.S155913. eCollection 2018.
Inorganic materials are widely used in medical devices, such as artificial hearts, vessels, and joints, in stents, and as nanocarriers for drug-delivery systems. Carbon nanomaterials are of particular interest due to their biological inertness and their capability to accommodate molecules. Several attempts have been proposed, in which carbon nanomaterials are used as nanocarriers for the systemic delivery of drugs.
We developed a drug-delivery system in which oxidized single-walled carbon nanohorns (oxSWNHs) were immobilized on a titanium (Ti) surface using material-binding peptides to enable localized drug delivery. For this purpose, we utilized a bispecific peptidic aptamer comprising a core sequence of a Ti-binding peptide and a SWNH-binding peptide to immobilize oxSWNHs on Ti.
Scanning electron microscopy was used to confirm the presence of oxSWNHs adsorbed onto the Ti surface, and a quartz crystal microbalance was used to evaluate the binding process during oxSWNH adsorption. The oxSWNHs-ornamented Ti substrate was nontoxic to cells and released biologically active dexamethasone over a sustained period.
This oxSWNHs-immobilized system can be used to modify the surface of Ti in implants and be loaded with drugs that stimulate osteogenesis and bone regeneration.
无机材料广泛应用于医疗器械，如人工心脏、血管和关节、支架以及作为药物输送系统的纳米载体。由于其生物惰性和容纳分子的能力，碳纳米材料尤其受到关注。已经提出了几种尝试，其中碳纳米材料被用作全身性药物输送的纳米载体。
我们开发了一种药物输送系统，其中氧化单壁碳纳米角（oxSWNH）通过材料结合肽固定在钛（Ti）表面上，以实现局部药物输送。为此，我们利用包含 Ti 结合肽和 SWNH 结合肽核心序列的双特异性肽适体将 oxSWNH 固定在 Ti 上。
扫描电子显微镜用于证实吸附在 Ti 表面上的 oxSWNHs 的存在，并且石英晶体微天平用于评估 oxSWNH 吸附过程中的结合过程。经过修饰的 Ti 基底表面固定 oxSWNHs 后，对细胞无毒，并且可以持续释放具有生物活性的地塞米松。
这种 oxSWNH 固定系统可用于修饰植入物中的 Ti 表面，并可装载刺激成骨和骨再生的药物。
