Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Queensland, Brisbane, Queensland, Australia.
Nat Protoc. 2011 Jan;6(1):36-45. doi: 10.1038/nprot.2010.171. Epub 2010 Dec 9.
Introducing bioactive molecules into plants helps establish their roles in plant growth and development. Here we describe a simple and effective petiole-feeding protocol to introduce aqueous solutions into the vascular stream and apoplast of dicotyledonous plants. This 'intravenous feeding' procedure has wide applicability to plant physiology, specifically with regard to the analysis of source-sink allocations, long-distance signaling, hormone biology and overall plant development. In comparison with existing methods, this technique allows the continuous feeding of aqueous solutions into plants without the need for constant monitoring. Findings are provided from experiments using soybean plants fed with a range of aqueous solutions containing tracer dyes, small metabolites, radiolabeled chemicals and biologically active plant extracts controlling nodulation. Typically, feeding experiments consist of (i) generating samples to feed (extracts, solutions and so on); (ii) growing recipient plants; (iii) setting up the feeding apparatus; and (iv) feeding sample solutions into the recipient plants. When the plants are ready, the feeding procedure can take 1-3 h to set up depending on the size of experiment (not including preparation of materials). The petiole-feeding technique also works with other plant species, including tomato, chili pepper and cabbage plants, as demonstrated here.
将生物活性分子引入植物有助于确定它们在植物生长和发育中的作用。在这里，我们描述了一种简单而有效的叶柄喂养方案，可将水溶液引入双子叶植物的维管流和质外体。这种“静脉内喂养”程序广泛适用于植物生理学，特别是在源库分配、长距离信号转导、激素生物学和整体植物发育的分析方面。与现有方法相比，该技术允许持续向植物中喂养水溶液，而无需进行持续监测。我们提供了使用大豆植物进行的一系列实验结果，这些实验使用了含有示踪染料、小代谢物、放射性标记化学物质和控制结瘤的生物活性植物提取物的各种水溶液进行喂养。通常，喂养实验包括：(i)生成要喂养的样品（提取物、溶液等）；(ii)培养受体植物；(iii)设置喂养装置；以及 (iv)将样品溶液喂养到受体植物中。当植物准备好后，根据实验的规模（不包括材料准备），设置喂养程序可能需要 1-3 小时。叶柄喂养技术也适用于其他植物物种，如番茄、辣椒和白菜，这里也进行了演示。
