Department of Horticulture and Crop Science, The Ohio State University, Wooster, OH 44691, USA.
Genome. 2012 Feb;55(2):140-6. doi: 10.1139/g11-086. Epub 2012 Jan 25.
The short petiole trait is valuable for the development of plant ideotypes with high yield by improving the plant canopy. The soybean breeding line SS98206SP has shown extremely short petioles in the greenhouse and fields. A new single recessive gene designated as lps3 confers the short petiole trait in SS98206SP. The objectives of this study were to map the short petiole gene in SS98206SP with PCR-based markers. In total, 187 F(2) plants and their F(2:3) families from a cross between the short petiole line SS98206SP and the long petiole cultivar 'Taekwang' along with the two parental lines were evaluated for their petiole lengths in a greenhouse. An SSR marker from each 10-cM section of a consensus soybean map was selected for bulked segregant analysis (BSA) to identify the tentative genomic location of the gene. The BSA technique was useful to localize the gene to a genomic region in the soybean linkage group F (chromosome 13). A linkage map with six SSR and two SNP markers flanking the gene was constructed. We positioned the gene between two SSR markers, Sat_234 and Sct_033, at distances of 8.5 and 3.5 cM from the marker, respectively. The makers flanking the gene (lps3) were located within 3-4 cM of the gene. These markers will be useful for maker-assisted selection in the development of new ideotype soybean plants.
短叶柄性状对于通过改善冠层来提高植物产量的理想株型的发展非常有价值。大豆品系 SS98206SP 在温室和田间表现出极短的叶柄。一个新的隐性单基因命名为 lps3，赋予 SS98206SP 短叶柄性状。本研究的目的是利用基于 PCR 的标记对 SS98206SP 中的短叶柄基因进行定位。总共评估了 187 株 F2 代植株及其 F2:3 代家系，这些植株来自短叶柄系 SS98206SP 与长叶柄品种“Taekwang”之间的杂交，以及两个亲本系，在温室中测量它们的叶柄长度。从大豆共识图谱的每个 10-cM 区段选择一个 SSR 标记进行混池分离分析（BSA），以鉴定基因的暂定基因组位置。BSA 技术有助于将基因定位到大豆连锁群 F（第 13 号染色体）中的一个基因组区域。构建了一个包含六个 SSR 和两个 SNP 标记的连锁图谱，该基因侧翼的标记。我们将基因定位在两个 SSR 标记 Sat_234 和 Sct_033 之间，距离标记分别为 8.5 和 3.5 cM。基因侧翼的标记（lps3）位于基因 3-4 cM 范围内。这些标记将有助于新理想株型大豆植物的标记辅助选择。
