Reutov V P, Azhipa Ia I, Kaiushin L P
Biull Eksp Biol Med. 1978 Sep;86(9):299-301.
A method of electron paramagnetic resonance was applied to the study of a possibility of paramagnetic centres formation in the nitrogen oxide reaction with saturated and unsaturated fatty acids and aromatic amino acids. Several paramagnetic centres are formed in interaction of NO2 with oleic acid at 20 degrees C. Saturated fatty acids formed no paramagnetic centres in the reaction with NO2. NO formed no paramagnetic centres not only with butyric, palmitic, and stearic acids, but also with oleic acid. The capacity of NO2 to form paramagentic centres with tyrosine in its saturated solution was revealed. In case of interaction between NO and NO2 with saturated phenylalanine and tryptophane solutions no formation of paramagnetic centres was observed. It is suggested that unsaturated fatty acids and tyrosine remnants of the membrane lipoprotein complexes could serve as peculiar targets with which NO2 interacted, and that formation of paramagnetic centres in the NO2 reaction with the mentioned substances was the primary act of the membrane damage.
应用电子顺磁共振方法研究了氮氧化物与饱和及不饱和脂肪酸以及芳香族氨基酸反应中顺磁中心形成的可能性。在20℃时，NO₂与油酸相互作用形成了几个顺磁中心。饱和脂肪酸在与NO₂反应中未形成顺磁中心。NO不仅与丁酸、棕榈酸和硬脂酸反应未形成顺磁中心，与油酸反应也未形成顺磁中心。研究发现，NO₂在其饱和溶液中能与酪氨酸形成顺磁中心。在NO和NO₂与饱和苯丙氨酸和色氨酸溶液相互作用的情况下，未观察到顺磁中心的形成。有人提出，膜脂蛋白复合物中的不饱和脂肪酸和酪氨酸残基可能是NO₂与之相互作用的特殊靶点，并且NO₂与上述物质反应形成顺磁中心是膜损伤的主要行为。
