Pharm Biol. 2000;38(1):25-9. doi: 10.1076/1388-0209(200001)3811-BFT025.
The influences of medium type, inoculum density, and a cold incubation on antimicrobial assay sensitivity were tested. The largest and most distinct zones were produced using nutrient agar and the 1/10 4 inoculum density for Staphylococcus aureus and Proteus mirabilis but a 1/10 12 inoculum density for Pseudomonas aeruginosa and Escherichia coli . The greatest number of zones were detected without cold incubation. Using this method, eight plants from Belize were screened for antibacterial activity. Six plants showed activity against the four organisms tested. Both inoculum density and medium type played important roles in assay sensitivity; however, inoculum density was of more practical significance.
测试了培养基类型、接种密度和冷藏对抑菌测定敏感性的影响。对于金黄色葡萄球菌和奇异变形杆菌，使用营养琼脂和 1/10^4 接种密度可产生最大和最明显的区域，而对于铜绿假单胞菌和大肠杆菌则使用 1/10^12 接种密度。在不进行冷藏的情况下，检测到的区域数量最多。使用这种方法，对来自伯利兹的 8 种植物进行了抗菌活性筛选。有 6 种植物对测试的 4 种生物表现出活性。接种密度和培养基类型对测定敏感性都有重要作用；但是，接种密度更具有实际意义。
