[新疆维吾尔族人群中血栓素合酶基因Rs10487667多态性与心肌梗死的相关性]

[Association of Rs10487667 genetic polymorphism of thromboxane synthase with myocardial infarction in Uigur population of Xinjiang].

作者信息

Wang Bao-zhu, Ma Yi-tong, Fu Zhen-yan, Xie Xiang, Zhang Xue-lian, Chen Bang-dang, Liu Fen, Yu Zi-xiang

机构信息

Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, Urumqi, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2010 Nov;44(11):1032-6.

PMID:21215134

Abstract

OBJECTIVE

To investigate the association between the polymorphism of thromboxane synthase gene (CYP5A1) and myocardial infarction (MI) of Uigur nationality patients in Xinjiang.

METHODS

Rs10487667 site polymorphism in CYP5A1 gene of 318 patients with MI (MI group) and 232 healthy control subjects (control group) were analyzed by polymerase chain reaction and restriction fragment length polymorphism. The serum thromboxane B(2)(TXB(2)) concentration was also detected in all subjects. The relationship of multiple factors and myocardial infarction was evaluated comprehensively by non-condition logistic regression analysis.

RESULTS

The frequencies of CYP5A1 gene Rs10487667 site polymorphism in MI group and control group were: GG type 0.204 (65/318) and 0.155 (36/232), GT type 0.553 (176/318) and 0.466 (106/232), TT type 0.242 (77/318) and 0.379 (88/232), respectively. There was significant difference in frequencies of GG genotype (χ(2) = 12.193, P = 0.002) between two groups and G allele frequency in MI group (0.481 (306/636)) was significant higher than control group (0.388 (180/464)) (χ(2) = 9.449, P = 0.021), but no difference in frequencies of GT and TT genotypes (χ(2) = 0.699, P > 0.05)between controls and MI cases. There was significant difference in serum TXB(2) level between MI ((184.3 ± 34.7) pg/ml) and control ((124.3 ± 28.1) pg/ml) groups (t = 5.503, P = 0.034). In the case and control group, the serum TXB(2) level of the person with GT + GG genotype ((164.21 ± 22.56) and (134.26 ± 19.83) pg/ml)) was significant higher than those of TT genotypes ((113.67 ± 54.23) and (98.54 ± 13.11) pg/ml) (t values were 5.433 and 5.108, respectively, both P values < 0.05). Logistic regression analysis showed that the T allele of the CYP5A1 gene was one independent risk factor of MI (OR = 1.673, 95%CI: 1.020 - 2.156) after adjustment of risk factors.

CONCLUSION

Rs10487667 polymorphism in CYP5A1 gene might be a risk factor of MI in Uigur population in Xinjiang, which maybe related with the significant high serum TXB(2) level.

摘要

目的

探讨血栓素合酶基因（CYP5A1）多态性与新疆维吾尔族心肌梗死（MI）患者的相关性。

方法

采用聚合酶链反应和限制性片段长度多态性技术，分析318例MI患者（MI组）和232例健康对照者（对照组）CYP5A1基因的Rs10487667位点多态性。同时检测所有研究对象血清血栓素B2（TXB2）浓度。采用非条件logistic回归分析综合评估多因素与心肌梗死的关系。

结果

MI组和对照组CYP5A1基因Rs10487667位点多态性的基因型频率分别为：GG型0.204（65/318）和0.155（36/232），GT型0.553（176/318）和0.466（106/232），TT型0.242（77/318）和0.379（88/232）。两组GG基因型频率差异有统计学意义（χ2 = 12.193，P = 0.002），MI组G等位基因频率为0.481（306/636），显著高于对照组的0.388（180/464）（χ2 = 9.449，P = 0.021），但GT和TT基因型频率差异无统计学意义（χ2 = 0.699，P > 0.05）。MI组（（184.3 ± 34.7）pg/ml）与对照组（（124.3 ± 28.1）pg/ml）血清TXB2水平差异有统计学意义（t = 5.503，P = 0.034）。病例组和对照组中，GT + GG基因型者血清TXB2水平（（164.21 ± 22.56）和（134.26 ± 19.83）pg/ml）显著高于TT基因型者（（113.67 ± 54.23）和（98.54 ± 13.11）pg/ml）（t值分别为5.433和5.108，P值均< 0.05）。logistic回归分析显示，校正危险因素后，CYP5A1基因的T等位基因为MI的独立危险因素（OR = 1.673，95%CI：1.020 - 2.156）。