Sato Masahiko, Goto-Koshino Yuko, Kanemoto Hideyuki, Mochizuki Hiroyuki, Fujino Yasuhito, Ohno Koichi, Tsujimoto Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):166-9. doi: 10.1016/j.vetimm.2010.08.009. Epub 2011 Jan 8.
BCL6 is known to be a key molecule in germinal center (GC) formation of lymph nodes, and its expression profiles have been implicated in the prognosis of diffuse large B-cell lymphoma in humans. The present study was carried out to characterize canine BCL6 cDNA and to indicate the technical methods for detection of the BCL6 protein in dog tissues. The deduced amino acid sequence of canine BCL6 showed close homology to that of human BCL6 (96.3%), especially in the zinc-finger motifs and POZ (poxvirus and zinc finger) domain with complete identity. Immunoblot analysis of a canine lymph node with an anti-human BCL6 monoclonal antibody revealed a band of 80 kDa. Immunohistochemical staining using the same antibody produced positive reactions in the cells exclusively localized in the GC of a canine lymph node. This study will be useful for the molecular classification of canine B-cell lymphomas with different prognoses.
已知BCL6是淋巴结生发中心（GC）形成中的关键分子，其表达谱与人类弥漫性大B细胞淋巴瘤的预后有关。本研究旨在鉴定犬BCL6 cDNA，并指出检测犬组织中BCL6蛋白的技术方法。犬BCL6推导的氨基酸序列与人类BCL6显示出高度同源性（96.3%），特别是在锌指基序和POZ（痘病毒和锌指）结构域完全相同。用抗人BCL6单克隆抗体对犬淋巴结进行免疫印迹分析，显示出一条80 kDa的条带。使用相同抗体进行免疫组织化学染色，在犬淋巴结GC中特定位的细胞中产生阳性反应。本研究将有助于对具有不同预后的犬B细胞淋巴瘤进行分子分类。
