Fukuda T, Miki T, Yoshida T, Hatano M, Ohashi K, Hirosawa S, Tokuhisa T
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Oncogene. 1995 Oct 19;11(8):1657-63.
The chromosomal translocation involving 3q27 is often detected in human B-cell lymphomas, especially diffuse lymphomas with a large-cell component. The BCL6 gene has been isolated from the chromosomal breakpoint in these lymphomas. Here we cloned the murine BCL6 (mBCL6) cDNA from the muscle cDNA library using the human BCL6 (hBCL6) cDNA as a probe. The predicted amino acid sequence was 95% identical to that of hBCL6. It contains six repeats of the Krüppel-like zinc-finger motif that are completely identical to those of hBCL6, indicating that the BCL6 gene is well conserved between humans and mice. Expression of the mBCL6 gene was ubiquitously detected in adult mouse tissues including lymphatic organs. Furthermore, it was induced in lymphocytes activated with phorbol ester and Ca2+ ionophore within 30 min after stimulation. This induction was not inhibited by treatment of the cells with a protein synthesis inhibitor, cycloheximide. These results suggest that BCL6 plays a role in activated lymphocytes as an immediate early gene.
涉及3q27的染色体易位在人类B细胞淋巴瘤中经常被检测到，尤其是具有大细胞成分的弥漫性淋巴瘤。BCL6基因已从这些淋巴瘤的染色体断点处分离出来。在此，我们以人BCL6（hBCL6）cDNA为探针，从肌肉cDNA文库中克隆了小鼠BCL6（mBCL6）cDNA。预测的氨基酸序列与hBCL6的氨基酸序列有95%的同一性。它包含六个Krüppel样锌指基序重复序列，与hBCL6的完全相同，表明BCL6基因在人和小鼠之间高度保守。mBCL6基因的表达在包括淋巴器官在内的成年小鼠组织中普遍被检测到。此外，在用佛波酯和Ca2+离子载体激活的淋巴细胞中，刺激后30分钟内可诱导其表达。这种诱导不受蛋白质合成抑制剂环己酰亚胺处理细胞的抑制。这些结果表明，BCL6作为一个立即早期基因在活化的淋巴细胞中发挥作用。
