Enzyme and Microbial Biochemistry Lab, Department of Chemistry, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi-110 016, India.
Bioresour Technol. 2011 Mar;102(5):4281-4. doi: 10.1016/j.biortech.2010.12.040. Epub 2010 Dec 15.
A mercury resistant strain of Enterobacter sp. is reported. The strain exhibited a novel property of mercury bioaccumulation with simultaneous synthesis of mercury nanoparticles. The culture conditions viz. pH 8.0 and lower concentration of mercury promotes synthesis of uniform sized 2-5 nm, spherical and monodispersed intracellular mercury nanoparticles. The remediated mercury trapped in the form of nanoparticles is unable to vaporize back into the environment thus, overcoming the major drawback of mercury remediation process. The mercury nanoparticles were recoverable. The nanoparticles have been characterized by high resolution transmission electron microscopy, energy dispersive X-ray analysis, powder X-ray diffraction and atomic force microscopy. The strain can be exploited for metal bioaccumulation from environmental effluent and developing a green process for nanoparticles biosynthesis.
报道了一株耐汞的肠杆菌属（Enterobacter sp.）菌株。该菌株具有一种新的汞生物积累特性，同时合成汞纳米颗粒。培养条件为 pH8.0 和较低浓度的汞可促进 2-5nm、球形和单分散的、均匀大小的、胞内汞纳米颗粒的合成。以纳米颗粒形式固定的修复汞无法重新蒸发回环境中，从而克服了汞修复过程的主要缺点。纳米颗粒是可回收的。通过高分辨率透射电子显微镜、能量色散 X 射线分析、粉末 X 射线衍射和原子力显微镜对纳米颗粒进行了表征。该菌株可用于从环境废水中进行金属生物积累，并开发用于纳米颗粒生物合成的绿色工艺。
