Kasan H C, Stegmann P
Department of Microbiology, University of Witwatersrand, Johannesburg, South Africa.
Microbios. 1987;51(207):89-96.
Bacterial strains tolerant to the presence of 100 mg/l zinc ions were isolated from a water reclamation system. Each of the organisms were screened for their ability to accumulate zinc at the above mentioned concentration. The organism capable of maximum zinc accumulation was identified as an Enterobacter species and intracellular zinc deposition by this micro-organism was determined using energy dispersive X-ray analysis, transmission electron microscopy and a metal-staining technique at the light microscopy level. Further studies revealed that growth and glucose utilization by this isolate were inhibited in the presence of zinc, compared to a control culture grown in the absence of zinc.
从一个水回收系统中分离出了耐受100毫克/升锌离子存在的细菌菌株。对每一种微生物进行筛选，以检测它们在上述浓度下积累锌的能力。能够最大程度积累锌的微生物被鉴定为肠杆菌属物种，并通过能量色散X射线分析、透射电子显微镜以及光学显微镜水平的金属染色技术来确定该微生物细胞内的锌沉积情况。进一步的研究表明，与在无锌环境中生长的对照培养物相比，在有锌存在的情况下，该分离菌株的生长和葡萄糖利用受到了抑制。
