荧光假单胞菌信号肽酶II基因（lsp）及其侧翼基因的核苷酸序列。
Nucleotide sequence of the Pseudomonas fluorescens signal peptidase II gene (lsp) and flanking genes.
作者信息
Isaki L, Beers R, Wu H C
机构信息
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889-4799.
出版信息
J Bacteriol. 1990 Nov;172(11):6512-7. doi: 10.1128/jb.172.11.6512-6517.1990.
The lsp gene encoding prolipoprotein signal peptidase (signal peptidase II) is organized into an operon consisting of ileS and three open reading frames, designated genes x, orf149, and orf316 in both Escherichia coli and Enterobacter aerogenes. A plasmid, pBROC128, containing a 5.8-kb fragment of Pseudomonas fluorescens DNA was found to confer pseudomonic acid resistance on E. coli host cells and to contain the structural gene of ileS from P. fluorescens. In addition, E. coli strains carrying pBROC128 exhibited increased globomycin resistance. This indicated that the P. fluorescens lsp gene was present on the plasmid. The nucleotide sequences of the P. fluorescens lsp gene and of its flanking regions were determined. Comparison of the nucleotide sequences of the lsp genes in E. coli and P. fluorescens revealed two highly conserved domains in this enzyme. Furthermore, the five genes which constitute an operon in E. coli and Enterobacter aerogenes were found in P. fluorescens in the same order as in the first two species.
编码前脂蛋白信号肽酶（信号肽酶II）的lsp基因被组织成一个操纵子，该操纵子在大肠杆菌和产气肠杆菌中均由ileS和三个开放阅读框组成，这三个开放阅读框分别被命名为基因x、orf149和orf316。发现一个含有荧光假单胞菌5.8 kb DNA片段的质粒pBROC128能赋予大肠杆菌宿主细胞对假单胞菌酸的抗性，并且含有来自荧光假单胞菌的ileS结构基因。此外，携带pBROC128的大肠杆菌菌株对球霉素的抗性增强。这表明荧光假单胞菌的lsp基因存在于该质粒上。测定了荧光假单胞菌lsp基因及其侧翼区域的核苷酸序列。对大肠杆菌和荧光假单胞菌中lsp基因的核苷酸序列进行比较，发现该酶中有两个高度保守的结构域。此外，在荧光假单胞菌中发现了与大肠杆菌和气肠杆菌中构成一个操纵子的五个基因，其排列顺序与前两个物种中的相同。
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