Mulligan M E, Hawley D K, Entriken R, McClure W R
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):789-800. doi: 10.1093/nar/12.1part2.789.
We describe a simple algorithm for computing a homology score for Escherichia coli promoters based on DNA sequence alone. The homology score was related to 31 values, measured in vitro, of RNA polymerase selectivity, which we define as the product KBk2, the apparent second order rate constant for open complex formation. We found that promoter strength could be predicted to within a factor of +/-4.1 in KBk2 over a range of 10(4) in the same parameter. The quantitative evaluation was linked to an automated (Apple II) procedure for searching and evaluating possible promoters in DNA sequence files.
我们描述了一种仅基于DNA序列来计算大肠杆菌启动子同源性得分的简单算法。该同源性得分与31个体外测量的RNA聚合酶选择性值相关,我们将其定义为产物KBk2,即开放复合物形成的表观二级速率常数。我们发现,在相同参数10⁴的范围内,启动子强度在KBk2中可以预测到±4.1倍的误差范围内。这种定量评估与一种自动化(苹果II型计算机)程序相关联,用于在DNA序列文件中搜索和评估可能的启动子。