Cetinkaya Mustafa Alp, Baydan Emine
National Food Reference Laboratory, Turkish Ministry of Agriculture and Rural Affairs, Fatih Sultan Mehmet Bulvari, Yenimahalle, Ankara, Turkey.
Interdiscip Toxicol. 2010 Mar;3(1):35-9. doi: 10.2478/v10102-010-0002-6. Epub 2010 Mar 29.
A range of pesticides is widely used in pest management and the chances of exposure to multiple organophosphorus (OP) compounds simultaneously are high, especially from dietary and other sources. Although health hazards of individual OP insecticides have been relatively well characterized, there is lesser information on the interactive toxicity of multiple OP insecticides. The aim of this study is to elicit the possible interactions in case combined exposure of an OP pesticide chlorpyrifos (CPF) and a plant growth regulator ethephon (ETF) which are used worldwide. The ileum segments of 3 months old Wistar Albino male rats were used in isolated organ bath containing Tyrode solution. ETF and CPF were incubated (10(-7) M concentration) separately or in combination with each other to ileum and their effects on acetylcholine-induced contractions were studied. The data obtained from this study show that, single and combined exposure to the agents caused agonistic interactions with regard to potency of ACh whereas they caused a decrease on E(max) value of ACh. These findings suggest that exposure to these agents which have direct and indirect cholinergic effects, may cause developing clinical responses with small doses and earlier but the extent of toxicity will be lower.
一系列农药广泛用于害虫治理，同时接触多种有机磷（OP）化合物的可能性很高，尤其是通过饮食和其他来源。尽管单个OP杀虫剂对健康的危害已得到相对充分的表征，但关于多种OP杀虫剂的联合毒性信息较少。本研究的目的是探讨在全球范围内使用的OP农药毒死蜱（CPF）和植物生长调节剂乙烯利（ETF）联合暴露时可能存在的相互作用。使用3个月大的Wistar白化雄性大鼠的回肠段，置于含有台氏液的离体器官浴中。将ETF和CPF（浓度为10⁻⁷ M）分别或相互组合孵育于回肠，研究它们对乙酰胆碱诱导的收缩的影响。本研究获得的数据表明，单独和联合暴露于这些药剂会导致在乙酰胆碱效力方面产生激动性相互作用，而它们会使乙酰胆碱的E(max)值降低。这些发现表明，接触这些具有直接和间接胆碱能作用的药剂，可能会导致小剂量且更早出现临床反应，但毒性程度会更低。
