Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Haweolgog-Dong, Sungbook-Gu, Seoul 136-791, South Korea.
Bioconjug Chem. 2011 Feb 16;22(2):125-31. doi: 10.1021/bc1004119. Epub 2011 Jan 10.
We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment.
我们报告了一种基于聚合物纳米颗粒平台设计的新型细胞凋亡纳米探针（Apo-NP）。这种简单的一步法技术能够在活细胞发生凋亡时增强荧光信号，从而实现单细胞和体内凋亡的实时成像。Apo-NP 能够有效地将化学标记的、双猝灭的 caspase-3 敏感型荧光肽递送到细胞内，允许 caspase-3 依赖性强荧光扩增在凋亡细胞中实时和高分辨率成像。Apo-NP 的设计平台具有灵活性，可以针对广泛的应用进行微调，例如鉴定病理学中的 caspase 相关凋亡，以及监测癌症治疗中凋亡药物的治疗效果。
