Shu Zhang, Xiaochen Liu, Chuyun Deng, Man Han, Juhua Pan, Xinghua Liao, Xin Qi, Shaojin Duan, Wanyun Ma
State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing, 100084, China.
J Fluoresc. 2014 Jul;24(4):1055-9. doi: 10.1007/s10895-014-1384-1. Epub 2014 Apr 17.
Cytosolic pH changes in single thymocytes in the early stages of apoptosis were examined with spectral-crosstalk-improved three-channel real-time fluorescence imaging. Spectral crosstalk was greatly reduced by this improved image processing method. Compared with normal thymocytes, the improved image processing method showed that the cytosolic pH value of thymocytes undergoing apoptosis was lower. A slight diversity in the intracellular pH values of samples subjected to different drug treatments was also observed. This new image processing method is beneficial to the application of the three-channel real-time fluorescence imaging to biological processes.
采用光谱串扰改进的三通道实时荧光成像技术检测了凋亡早期单个胸腺细胞内的pH变化。通过这种改进的图像处理方法，光谱串扰大大降低。与正常胸腺细胞相比，改进的图像处理方法显示正在经历凋亡的胸腺细胞的胞质pH值较低。还观察到接受不同药物处理的样品在细胞内pH值上存在轻微差异。这种新的图像处理方法有利于三通道实时荧光成像在生物过程中的应用。
