ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
J Anal Toxicol. 2011 Jan;35(1):40-5. doi: 10.1093/anatox/35.1.40.
The results of meconium specimens and fortified samples screened for drugs of abuse by both enzyme-linked immunosorbent assay (ELISA, Immunalysis) and biochip microarray (Randox) methods were compared. The ELISA method was semi-automated using a TECAN Genesis. The Randox assay used the Randox Evidence Investigator system. Previously validated gas chromatography-mass spectrometry (GC-MS), GC-GC-MS, or liquid chromatography-MS-MS methods were used for confirmation and quantitation. Results from the two techniques compared well. Agreement of the Randox assay was greater than 90% when compared to the ELISA assay for all drug classes except cannabinoids (88%). Specificity of the biochip assay was slightly better for amphetamines and cocaine.
比较了酶联免疫吸附测定（ELISA，Immunalysis）和生物芯片微阵列（Randox）两种方法对粪便标本和强化样本进行药物滥用筛查的结果。ELISA 方法使用 TECAN Genesis 半自动进行。Randox 检测使用 Randox Evidence Investigator 系统。先前经过验证的气相色谱-质谱（GC-MS）、GC-GC-MS 或液相色谱-MS-MS 方法用于确认和定量。两种技术的结果比较吻合。除大麻素（88%）外，与 ELISA 检测相比，Randox 检测对所有药物类别均具有大于 90%的一致性。生物芯片检测对苯丙胺和可卡因的特异性略高。
