Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, ZC 91501-970, Brazil.
Bioresour Technol. 2011 Mar;102(5):4218-25. doi: 10.1016/j.biortech.2010.12.060. Epub 2010 Dec 22.
The production of ethanol by the new yeast Spathaspora arborariae using rice hull hydrolysate (RHH) as substrate, either alone or in co-cultures with Saccharomyces cerevisiae is presented. Cultivations were also carried out in synthetic medium to gather physiological information on these systems, especially concerning their ability to grow and produce ethanol in the presence of acetic acid, furfural, and hydroxymethylfurfural, which are toxic compounds usually present in lignocellulosic hydrolysates. S. arborariae was able to metabolize xilose and glucose present in the hydrolysate, with ethanol yields (Y(P/S)(et)) of 0.45. In co-cultures, ethanol yields peaked to 0.77 and 0.62 in the synthetic medium and in RHH, respectively. When the toxic compounds were added to the synthetic medium, their presence produced negative effects on biomass formation and ethanol productivity. This work shows good prospects for the use of the new yeast S. arborariae alone and in co-cultures with S. cerevisiae for ethanol production.
介绍了利用稻壳水解物（RHH）作为底物，通过新型酵母 Spathaspora arborariae 单独或与酿酒酵母共培养生产乙醇的情况。还在合成培养基中进行了培养，以收集这些系统的生理信息，特别是它们在存在乙酸、糠醛和羟甲基糠醛（通常存在于木质纤维素水解物中的有毒化合物）的情况下生长和生产乙醇的能力。S. arborariae 能够代谢水解物中的木糖和葡萄糖，乙醇得率（Y(P/S)(et)）为 0.45。在共培养物中，乙醇得率分别在合成培养基和 RHH 中达到 0.77 和 0.62 的峰值。当向合成培养基中添加有毒化合物时，它们的存在对生物量形成和乙醇生产力产生了负面影响。这项工作表明，新型酵母 S. arborariae 单独使用和与酿酒酵母共培养生产乙醇具有良好的前景。
