Beers W H, Strickland S, Reich E
Cell. 1975 Nov;6(3):387-94. doi: 10.1016/0092-8674(75)90188-9.
A technique is described for detecting fibrinolytic activity of single cells in culture. This method was applied to the analysis of rat ovarian granulosa cells. Cells obtained from follicules shortly before ovulation show high levels of fibrinolytic activity. This activity is plasminogen-dependent, indicating that it is due to plasminogen activator. The appearance of this activity is correlated with ovulation by temporal and functional criteria, and can be demonstrated both in immature animals primed with hormones and in mature cycling animals. Granulosa cell cultures can be stimulated to release plasminogen activator by exposure in vitro either to luteinizing hormone or to dibutyryl cyclic AMP.
本文描述了一种用于检测培养的单细胞纤溶活性的技术。该方法应用于大鼠卵巢颗粒细胞的分析。排卵前不久从卵泡中获得的细胞显示出高水平的纤溶活性。这种活性依赖于纤溶酶原，表明它是由纤溶酶原激活剂引起的。根据时间和功能标准，这种活性的出现与排卵相关，并且在激素预处理的未成熟动物和成熟的周期性动物中都可以得到证实。体外暴露于促黄体生成素或二丁酰环磷腺苷可刺激颗粒细胞培养物释放纤溶酶原激活剂。
