荧光硅纳米粒子的合成及其作为荧光探针的应用。

Synthesis of fluorescent silica nanoparticles and their applications as fluorescence probes.

机构信息

Department of Chemistry, Shanghai Normal University, Shanghai 200234, People's Republic of China.

出版信息

J Fluoresc. 2011 May;21(3):1205-12. doi: 10.1007/s10895-010-0799-6. Epub 2011 Jan 8.

Abstract

This paper presents the synthesis of organic dye molecules embedded silica nanoparticles by Stöber method and their applications as fluorescence probes in cell imaging. By modifying the surface of fluorescent silica nanoparticles (FSNs) with amino, biologically functionalized and monodisperse FSNs can be obtained. In this work, FSNs were conjugated with monoclonal anti-Carcinoembryonic Antigen (anti-CEA) antibody via covalent binding. The antibody-conjugated FSNs can be used to label the SPCA-1 cells successfully, demonstrating that the application of FSNs as fluorescence probes in fluorescence imaging and bioassay would be feasible.

摘要

本文通过 Stöber 方法合成了嵌入硅纳米粒子的有机染料分子，并将其作为荧光探针应用于细胞成像。通过对荧光硅纳米粒子（FSN）的表面进行修饰，可以得到具有氨基的、生物功能化的、单分散的 FSN。在这项工作中，FSN 通过共价键与单克隆抗癌胚抗原（anti-CEA）抗体偶联。抗体偶联的 FSN 可以成功标记 SPCA-1 细胞，这表明 FSN 作为荧光探针在荧光成像和生物测定中的应用是可行的。