富氢盐水对烫伤大鼠延迟复苏后血压及肺组织抗氧化能力的影响

[Effect of hydrogen-rich saline on blood pressure and antioxidant ability of lung tissue in scalded rats following delayed resuscitation].

作者信息

Qiu Xiao-Chen, Jin Yi-Chao, Sun Yu, Luo Peng-Fei, Fu Jin-Feng, Chen Bin, Xia Zhao-Fan

机构信息

Institute of Burn Research, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China.

出版信息

Zhonghua Shao Shang Za Zhi. 2010 Dec;26(6):435-8.

PMID:21223652

Abstract

OBJECTIVE

To study the effect of hydrogen-rich saline on blood pressure and antioxidant ability of lung tissue in scalded rats following delayed resuscitation.

METHODS

The hydrogen-rich saline was prepared (hydrogen-saturated normal saline with hydrogen concentration of 0.6 mmol/L). Twenty SD rats were divided into hydrogen-rich saline group (HS) and normal saline group (NS) according to the random number table, with 10 rats in each group. All the rats were subjected to 30% total body surface area (TBSA) full-thickness scald. Rats in HS and NS groups were infused with hydrogen-rich saline or normal saline with one half of the total fluid replacement volume as calculated according to the Parkland formula (4 mL×kg(-1)×%TBSA(-1)) at post scald hour (PSH) 7 and one-quarter of the volume at PSH 9 and 17 respectively. The general condition of rats during the experiment was observed. The systolic pressure of rats was measured at PSH 6 and 24. All rats were sacrificed at PSH 24 to collect lung tissue for detecting superoxide dismutase (SOD) inhibition ratio and malondialdehyde (MDA) level. Data were processed with t test.

RESULTS

All rats survived through the experiment. The systolic pressure of rats in HS group and NS group was respectively (87 ± 4) mm Hg (1 mm Hg = 0.133 kPa) and (86 ± 5) mm Hg at PSH 6, and the values were close (t = 0.213, P = 0.834); however the systolic pressure at 24 h was higher in HS group than in NS group [(124 ± 7) mm Hg vs. (115 ± 6) mm Hg, t = 2.958, P = 0.008]. SOD inhibition ratio of lung tissue in HS group [(0.465 ± 0.014)%] was higher than that in NS group [(0.358 ± 0.021)%, t = 11.767, P = 0.000]. MDA level of lung tissue in HS group [(922 ± 196) pmol/mg] was lower than that in NS group [(1118 ± 212) pmol/mg, t = -2.142, P = 0.046].

CONCLUSIONS

Delayed resuscitation for scalded rats with hydrogen-rich saline is helpful in the recovery of systolic pressure, and it can ameliorate lung tissue injury caused by reperfusion through enhancing the effect of antioxidase.

摘要

目的

研究富氢盐水对烫伤大鼠延迟复苏后血压及肺组织抗氧化能力的影响。

方法

制备富氢盐水（氢气饱和生理盐水，氢气浓度为0.6 mmol/L）。将20只SD大鼠按随机数字表法分为富氢盐水组（HS）和生理盐水组（NS），每组10只。所有大鼠均行30%总体表面积（TBSA）全层烫伤。HS组和NS组大鼠分别于烫伤后7小时（PSH 7）按Parkland公式计算的总补液量的一半（4 mL×kg⁻¹×%TBSA⁻¹）输注富氢盐水或生理盐水，于PSH 9和17分别输注四分之一的量。观察实验期间大鼠的一般情况。于PSH 6和24测量大鼠收缩压。所有大鼠于PSH 24处死，取肺组织检测超氧化物歧化酶（SOD）抑制率和丙二醛（MDA）水平。数据采用t检验处理。

结果

所有大鼠均存活至实验结束。HS组和NS组大鼠在PSH 6时收缩压分别为（87±4）mmHg（1 mmHg = 0.133 kPa）和（86±5）mmHg，两者相近（t = 0.213，P = 0.834）；但HS组24小时收缩压高于NS组[（124±7）mmHg对（115±6）mmHg，t = 2.958，P = 0.008]。HS组肺组织SOD抑制率[（0.465±0.014）%]高于NS组[（0.358±0.021）%，t = 11.767，P = 0.000]。HS组肺组织MDA水平[（922±196）pmol/mg]低于NS组[（1118±212）pmol/mg，t = -2.142，P = 0.046]。