Sha Hui-Lan, Ouyang Wei-Xiang, Lü Gang
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhonghua Zhong Liu Za Zhi. 2010 Dec;32(12):913-6.
To detect the expression of Toll-like receptor 9 (TLR9) in ovarian cancer, and to explore their clinical significance.
Western blot method and immunohistochemical staining were used to examine the expression of TLR9 in the ovarian cancer, paracancerous tissues and normal ovarian tissues, obtained during operation from 30 ovarian carcinoma patients and 30 normal non-tumor patients. The relationships of TLR9 with pathological grade, clinical stage, and metastasis of ovarian cancer were statistically analyzed.
The percentage of positive cells expressing TLR9 protein in human ovarian cancer tissues, paracancerous tissues and normal ovarian tissues were 80.0%, 36.7% and 20.0%, respectively. The protein expression level of TLR9 was gradually descending (P < 0.01). The highly expressed TLR9 significantly correlated with the degree of tumor differentiation, an advanced FIGO stage and lymph node metastasis. The Western blot results showed that TLR9 protein expression in ovarian cancer, paracancerous tissues and normal ovarian tissues were 0.803 ± 0.072, 0.411 ± 0.087 and 0.113 ± 0.065, respectively. The expression of TLR9 in ovarian cancer was significantly higher than that in normal tissue and paracancerous tissues (P < 0.01).
TLR9 has a higher expression in ovarian cancer tissues. TLR9 expression has a close relationship with pathological grades of ovarian cancer, suggesting that TLR9 plays an important role in the development and progression of ovarian cancer through immunologic mechanisms.
检测Toll样受体9（TLR9）在卵巢癌中的表达，并探讨其临床意义。
采用蛋白质免疫印迹法和免疫组织化学染色法检测30例卵巢癌患者及30例正常非肿瘤患者手术中获取的卵巢癌组织、癌旁组织及正常卵巢组织中TLR9的表达情况。对TLR9与卵巢癌病理分级、临床分期及转移的关系进行统计学分析。
人卵巢癌组织、癌旁组织及正常卵巢组织中TLR9蛋白阳性细胞表达率分别为80.0%、36.7%和20.0%。TLR9蛋白表达水平呈逐渐下降趋势（P < 0.01）。TLR9高表达与肿瘤分化程度、国际妇产科联盟（FIGO）分期及淋巴结转移显著相关。蛋白质免疫印迹结果显示，卵巢癌组织、癌旁组织及正常卵巢组织中TLR9蛋白表达量分别为0.803±0.072、0.411±0.087和0.113±0.065。卵巢癌中TLR9的表达显著高于正常组织和癌旁组织（P < 0.01）。
TLR9在卵巢癌组织中表达较高。TLR9表达与卵巢癌病理分级密切相关，提示TLR9通过免疫机制在卵巢癌的发生发展中起重要作用。
