牛胎盘碳酸酐酶同工酶的免疫组织化学研究。

Immunohistochemical studies of the carbonic anhydrase isozymes in the bovine placenta.

作者信息

Nishita T, Kinoshita C, Maegaki M, Asari M

机构信息

Department of Physiology I, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.

出版信息

Placenta. 1990 Jul-Aug;11(4):329-36. doi: 10.1016/s0143-4004(05)80224-3.

DOI:10.1016/s0143-4004(05)80224-3

PMID:2122437

Abstract

Placentae from 11 cows, ranging from about 80 to 270 days gestation, were studied for immunohistochemical localization of carbonic anhydrase isozymes. CA isozymes were localized using the avidin-biotin-peroxidase complex (ABC) method in the bovine placenta. CA-II was found in the fetal trophoblastic cells with single nuclei as well as in erythrocytes of maternal and fetal blood. The percentage of positive cells for anti-CA-II in the trophoblastic cells did not change during development of gestation in the fetal bovine. CA-I and CA-III were not detected in the bovine placenta.

摘要

对11头处于约80至270天妊娠期的奶牛胎盘进行研究，以免疫组织化学方法定位碳酸酐酶同工酶。采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法在牛胎盘中定位碳酸酐酶同工酶。在单核的胎儿滋养层细胞以及母体和胎儿血液的红细胞中发现了碳酸酐酶II（CA-II）。在胎牛妊娠期发育过程中，滋养层细胞中抗CA-II阳性细胞的百分比没有变化。在牛胎盘中未检测到碳酸酐酶I（CA-I）和碳酸酐酶III（CA-III）。