Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.
Intervirology. 2011;54(5):282-9. doi: 10.1159/000319430. Epub 2011 Jan 13.
Partial genome sequences were determined and subjected to comparative analyses from two fish herpesviruses (HVs). Acipenserid (Aci) HV-2, originating from the white sturgeon (Acipenser transmontanus), and ictalurid (Ic) HV-2, isolated from the black bullhead (Ameiurus melas), are recently approved species of the genus Ictalurivirus of the family Alloherpesviridae.
An almost 8,000-base-pair fragment, spanning between the genes of the DNA polymerase and the ATPase subunit of the terminase, was sequenced from each virus.
The size, position and orientation of 2 partial and 3 full open reading frames, contained in the studied genome fragment, proved to be similar to their counterparts in IcHV-1, the type species of the genus Ictalurivirus. Thus, a well-conserved genus-specific gene block was identified. In the members of two other genera (Cyprinivirus and Batrachovirus) of the family Alloherpesviridae, no such gene block could be found; the location and orientation of the homologous genes showed significant divergence.
The results of phylogenetic calculations were in good agreement with the genome arrangements inasmuch as AciHV-2, IcHV-1 and -2 are monophyletic and separated from the lineages of the other two genera. The new sequence enabled the inclusion of a hitherto unassigned HV, that of the Australian pilchard, into a phylogenetic calculation.
测定并比较两种鱼类疱疹病毒（HV）的部分基因组序列。白鲟 HV-2 来源于白鲟（Acipenser transmontanus），而美洲鳗 HV-2 则分离自黑鲈（Ameiurus melas），它们是新近被批准的疱疹病毒属成员，归为疱疹病毒科的 Alloherpesviridae 科。
对来自这两种病毒的约 8000 个碱基对的片段进行测序，该片段跨越 DNA 聚合酶和末端酶 ATP 亚基基因。
在所研究的基因组片段中，2 个部分和 3 个完整开放阅读框的大小、位置和方向与疱疹病毒属的 IcHV-1 （疱疹病毒属的模式种）的对应部分相似。因此，确定了一个高度保守的属特异性基因块。在疱疹病毒科的另外两个属（鲤鱼病毒属和蛙病毒属）的成员中，找不到这样的基因块；同源基因的位置和方向显示出明显的差异。
由于 AciHV-2、IcHV-1 和 -2 是单系的，与其他两个属的谱系分离，因此系统发育计算的结果与基因组排列情况一致。新序列使我们能够将迄今为止未分配的 HV，即澳大利亚沙丁鱼 HV，纳入系统发育计算。
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