Voloshchuk T P, Patskovskiĭ Iu V, Potopal'skiĭ A I
Bioorg Khim. 1990 Jul;16(7):981-90.
In the course of investigating the reaction conditions of the nucleic acid components alcylation, the interaction of thioTEPA (N,N',N''-triethylenethiophosphoamide) with hydrochloric and perchloric acids was studied, perchloric acid increasing the alkylation products yield. HPLC and UV spectroscopy were used to isolate and identify products of nucleic bases alkylation by ethylenimine and its derivatives (thioTEPA and monoaziridinediethylphosphate). It is shown that under neutral conditions phosphoaminoethylation takes place, whereas under slightly acidic conditions products of aminoethylation are formed.
在研究核酸成分烷基化反应条件的过程中，研究了硫替派（N,N',N''-三乙烯硫代磷酰胺）与盐酸和高氯酸的相互作用，高氯酸可提高烷基化产物的产率。采用高效液相色谱法（HPLC）和紫外光谱法分离并鉴定了乙撑亚胺及其衍生物（硫替派和磷酸单氮丙啶二乙酯）对核酸碱基的烷基化产物。结果表明，在中性条件下发生磷氨基乙基化，而在弱酸性条件下形成氨基乙基化产物。
