Barnes J L, Goñi F, Heyermann H, Frangione B, Agnello V
Department of Laboratory Medicine, Lahey Clinic Medical Center, Burlington, Massachusetts 01805.
Arthritis Rheum. 1990 Nov;33(11):1710-5. doi: 10.1002/art.1780331115.
The BLA cross-idiotype (XId) is present on a unique subset of rheumatoid factors (RF) that cross-react with DNA-histone. In this study, prototype Bla monoclonal RF was shown from serologic investigations and N-terminal amino acid sequence analysis to have distinct kappa chains related to the V kappa III subgroup and VH4 heavy chains. The amino terminus of the heavy chain was cyclized, rendering the protein resistant to Edman degradation and providing a possible investigator bias to the published Ig sequence data to date. This appears to be the first definitive report of a serum IgM that expresses the VH4 gene. RF with DNA cross-reactivity have been reported to be produced by human and mouse cloned cells that have the VH4 or homologous mouse Vh36-60 gene.
BLA交叉独特型（XId）存在于与DNA-组蛋白发生交叉反应的类风湿因子（RF）的一个独特亚群上。在本研究中，通过血清学研究和N端氨基酸序列分析表明，原型Bla单克隆RF具有与VκIII亚群相关的独特κ链和VH4重链。重链的氨基末端被环化，使该蛋白对埃德曼降解具有抗性，并可能导致了迄今为止已发表的Ig序列数据存在研究者偏差。这似乎是关于表达VH4基因的血清IgM的第一份确切报告。据报道，具有DNA交叉反应性的RF是由具有VH4或同源小鼠Vh36-60基因的人和小鼠克隆细胞产生的。
