Wang K Q, Li Z G, Hao Q L, He J L, Li X Z, Zhang H Z, Tang J J, Wu G, Chen B S, Wang J M
Institute of Basic Medical Sciences, CAMS, Beijing.
Proc Chin Acad Med Sci Peking Union Med Coll. 1990;5(2):112-9.
The differences of serum lipid and lipoprotein (LP, profiles of animals susceptible (rabbit) and nonsusceptible (Beijing duck) to atherosclerosis as well as the distribution of apolipoprotein (apo) A-I and its catabolism in vivo were studied. Eight items, i.e. total cholesterol (TC), high density lipoprotein (HDL) cholesterol, triglyceride, percentage of alpha-LP and beta-LP, beta-LP concentration, lecithin cholesterol acyl transferase (LCAT), and agarose and polyacrylamide gel electrophoresis of both species were assayed before and after feeding a high fat, high cholesterol diet. Results indicate that the exogenous cholesterol consumed by Beijing ducks was carried and transported by HDL, while that in rabbits was transported by low density lipoprotein (LDL). The biological half lives of apo A-I in serum and in HDL were 41.1 +/- 2.4 and 42.8 +/- 1.7 h respectively, and its distribution in different organs was in the order of liver greater than kidney greater than spleen greater than lung greater than heart greater than intestine greater than muscles greater than aorta. These results show that the liver is the major organ for metabolizing HDL apo A-I, and the kidney is also important. The results also imply that in Beijing ducks the cholesterol carried by HDL may be catabolized through apo A-I receptors in the liver and kidney. The differences in cholesterol binding and in lipoprotein and apolipoprotein metabolism of the two species provide important clues for the elucidation of the pathogenesis of atherosclerosis.
研究了易患动脉粥样硬化的动物（兔）和不易患动脉粥样硬化的动物（北京鸭）的血脂和脂蛋白（LP）谱差异，以及载脂蛋白（apo）A-I在体内的分布及其分解代谢。在给予高脂、高胆固醇饮食前后，对两种动物的八项指标进行了检测，即总胆固醇（TC）、高密度脂蛋白（HDL）胆固醇、甘油三酯、α-LP和β-LP的百分比、β-LP浓度、卵磷脂胆固醇酰基转移酶（LCAT），以及琼脂糖和聚丙烯酰胺凝胶电泳。结果表明，北京鸭摄入的外源性胆固醇由HDL携带和运输，而兔体内的外源性胆固醇则由低密度脂蛋白（LDL）运输。apo A-I在血清和HDL中的生物半衰期分别为41.1±2.4小时和42.8±1.7小时，其在不同器官中的分布顺序为肝脏＞肾脏＞脾脏＞肺＞心脏＞肠道＞肌肉＞主动脉。这些结果表明，肝脏是代谢HDL apo A-I的主要器官，肾脏也很重要。结果还表明，在北京鸭中，HDL携带的胆固醇可能通过肝脏和肾脏中的apo A-I受体进行分解代谢。两种动物在胆固醇结合以及脂蛋白和载脂蛋白代谢方面的差异为阐明动脉粥样硬化的发病机制提供了重要线索。
