College of Life Science, Ocean University of China, Qingdao, China.
Basic Clin Pharmacol Toxicol. 2011 Jun;108(6):390-5. doi: 10.1111/j.1742-7843.2011.00676.x. Epub 2011 Feb 14.
This study was designed to investigate the therapeutic effect of sodium formononetin-3'-sulphonate (Sul-F), a water-soluble derivate of formononetin, on acute myocardial infarction in rats. The results showed that treatment with Sul-F significantly prevented the elevation of ST-segment level, decreased the contents of creatine kinase-MB, lactate dehydrogenase, alanine aminotransferase and cardiac troponin T in serum and reduced the myocardium necrosis scores. The number of apoptosis cardiocytes is well accordance with the up-regulated expression of Bcl-2 and the down-regulated expression of Bax. Meanwhile, Sul-F significantly increased the cardiac mitochondrial ATP content, improved ATP synthase activity, decreased thiobarbituric acid-reactive substances content and attenuated the decrease in superoxide dismutase and glutathione peroxidase activities. These findings indicate that Sul-F has a protective potential against myocardial infarction injury. A possible mechanism for the protective effect is the elevated expression of endogenous antioxidant defence enzymes degraded lipid peroxidation products and improved energy metholism of cardiac mitochondrial, thus attenuating cardiocyte apoptosis.
本研究旨在探讨芒柄花黄素-3'-磺酸钠（Sul-F），一种芒柄花黄素的水溶性衍生物，对大鼠急性心肌梗死的治疗作用。结果表明，Sul-F 治疗可显著防止 ST 段抬高，降低血清肌酸激酶同工酶-MB、乳酸脱氢酶、丙氨酸氨基转移酶和心肌肌钙蛋白 T 的含量，降低心肌坏死评分。凋亡心肌细胞的数量与 Bcl-2 的上调表达和 Bax 的下调表达相吻合。同时，Sul-F 还显著增加了心肌线粒体的 ATP 含量，改善了 ATP 合酶的活性，降低了丙二醛含量，减轻了超氧化物歧化酶和谷胱甘肽过氧化物酶活性的降低。这些发现表明 Sul-F 对心肌梗死损伤具有保护作用。其保护作用的可能机制是内源性抗氧化防御酶表达的升高，降解脂质过氧化产物，改善心肌线粒体的能量代谢，从而减轻心肌细胞凋亡。
