Livestock Infectious Diseases Programme, Institute for Animal Health, Compton, Berkshire, RG20 7NN, UK.
Dev Comp Immunol. 2011 May;35(5):580-91. doi: 10.1016/j.dci.2011.01.001. Epub 2011 Jan 11.
Bovine tuberculosis (TB) is a disease of economic importance and a significant animal health and welfare issue. The alveolar macrophage (AlvMϕ) plays a vital role in the immune response to TB and recent studies provide insights into the interactions between Mϕ and Mycobacterium bovis. Here we reveal the early transcriptional response of bovine AlvMϕ to M. bovis infection. We demonstrate up-regulation of immune response genes, including chemokines, members of the NF-κB pathway which may be involved in their transcription and also pro- and anti-apoptotic genes. M. bovis may therefore induce multiple mechanisms to manipulate the host immune response. We compared the response of AlvMϕ to infection with live and heat-killed M. bovis to determine transcriptional differences dependent on the viable pathogen. Several chemokines up-regulated following live M. bovis infection were not up-regulated after heat-killed M. bovis stimulation; hence the Mϕ seems to differentiate between the two stimuli.
牛结核病（TB）是一种具有重要经济意义的疾病，也是一个重大的动物健康和福利问题。肺泡巨噬细胞（AlvMϕ）在对结核病的免疫反应中起着至关重要的作用，最近的研究深入了解了巨噬细胞（Mϕ）与牛分枝杆菌（Mycobacterium bovis）之间的相互作用。在这里，我们揭示了牛肺泡巨噬细胞（AlvMϕ）对牛分枝杆菌（M. bovis）感染的早期转录反应。我们证明了免疫反应基因的上调，包括趋化因子和 NF-κB 途径的成员，它们可能参与其转录，以及促凋亡和抗凋亡基因。因此，牛分枝杆菌（M. bovis）可能诱导多种机制来操纵宿主的免疫反应。我们比较了 AlvMϕ 对活的和热灭活的牛分枝杆菌（M. bovis）感染的反应，以确定依赖于活病原体的转录差异。在活的牛分枝杆菌（M. bovis）感染后上调的几种趋化因子在热灭活的牛分枝杆菌（M. bovis）刺激后没有上调；因此，巨噬细胞（Mϕ）似乎可以区分这两种刺激物。
