State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Insect Biochem Mol Biol. 2011 Apr;41(4):254-63. doi: 10.1016/j.ibmb.2011.01.002. Epub 2011 Jan 11.
Insect odorant binding proteins (OBPs) are required for insect olfaction perception and play a key role in transporting hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (Ors). We constructed two high-quality cDNA libraries from the male and female antennae of the lucerne plant bug, Adelphocoris lineolatus (Goeze) (Hemiptera: Miridae), a hemipteran species in a large and economically important phylogenetic group of phytophagous insects. A total of 1538 male ESTs and 1576 female ESTs were sequenced and analyzed, which produced 2915 high-quality ESTs for further analysis. The 2915 ESTs were assembled to 1423 unigenes. Of the 1423 unigenes, 895 (63%) showed no significant similarity with any known GenBank entry. The most prevalent transcripts in the cDNA libraries are OBPs, chemosensory proteins (CSPs), protein takeout precursors, antennae-specific proteins. We identified 14 genes encoding 12 "classical OBPs" with only six conserved cysteines and 2 "Plus-C OBPs" with two additional conserved cysteines and a conserved proline immediately after the sixth cysteine. AlinOBP4 has a very high amino acid identity of 89% to LAP, a well studied OBP of the tarnished plant bug Lygus lineolaris. The expression profiles of the 14 OBPs in different tissues (antennae, heads, thoraxes, abdomens, legs and wings) were measured by real-time qPCR. The results revealed some OBP genes are highly and differentially expressed in male and female antennae, and four OBP genes have an extremely high transcript level in the legs. Of two Plus-C OBP genes, one (AlinOBP14) is highly expressed only in the heads and another (AlinOBP7) is expressed in all olfactory tissues with much lower transcript levels comparing to other OBPs. The possible physiological functions of these OBPs are discussed.
昆虫气味结合蛋白（OBPs）是昆虫嗅觉感知所必需的，在将疏水性信息素穿过感器淋巴转运到嗅觉受体（Ors）中起着关键作用。我们从苜蓿盲蝽（Adelphocoris lineolatus（Goeze））（半翅目：盲蝽科）的雄触角和雌触角构建了两个高质量的 cDNA 文库，这是一个在植食性昆虫的大型且具有重要经济意义的系统发育群中属于半翅目的物种。共测序和分析了 1538 个雄性 EST 和 1576 个雌性 EST，共产生了 2915 个高质量的 EST 进行进一步分析。2915 个 EST 组装成 1423 个 Unigenes。在 1423 个 Unigenes中，895 个（63％）与任何已知的 GenBank 条目没有显著相似性。cDNA 文库中最常见的转录本是 OBPs、化学感觉蛋白（CSPs）、蛋白取出前体、触角特异性蛋白。我们鉴定了 14 个基因，这些基因编码 12 个具有仅 6 个保守半胱氨酸的“经典 OBPs”和 2 个具有另外 2 个保守半胱氨酸和第六个半胱氨酸后面的保守脯氨酸的“Plus-C OBPs”。AlinOBP4 与 Tarnished 植食性盲蝽 Lygus lineolaris 中研究得很好的 OBP LAP 的氨基酸同一性非常高，为 89％。通过实时 qPCR 测量了 14 个 OBPs 在不同组织（触角、头部、胸部、腹部、腿部和翅膀）中的表达谱。结果表明，一些 OBP 基因在雄性和雌性触角中高度且差异表达，并且有 4 个 OBP 基因在腿部中具有极高的转录水平。在两个 Plus-C OBP 基因中，一个（AlinOBP14）仅在头部中高度表达，另一个（AlinOBP7）在所有嗅觉组织中表达，与其他 OBPs 相比转录水平要低得多。讨论了这些 OBPs 的可能生理功能。
