Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.
Trends Plant Sci. 2011 Mar;16(3):141-50. doi: 10.1016/j.tplants.2010.12.004. Epub 2011 Jan 11.
Although protein-protein interaction (PPI) networks have been shown to offer a systems-wide view of cellular processes, only a few plant PPI maps are available. Recently, the core cell cycle of Arabidopsis thaliana has been analyzed by three independent PPI technologies, including yeast two-hybrid systems, bimolecular fluorescence complementation and tandem affinity purification. Here, we merge the three interactomes with literature-curated and computationally predicted interactions, paving the way for a comprehensive picture of the plant core cell cycle machinery. Platform-specific interactions unveil the strengths and weaknesses of each detection method and give insights into the nature of the interactions among cell cycle proteins. Moreover, comparison of the obtained data reveals that a complete interactome can only be obtained when multiple techniques are applied in parallel.
尽管蛋白质-蛋白质相互作用（PPI）网络已经被证明可以提供细胞过程的系统范围视图，但只有少数植物 PPI 图谱可用。最近，通过三种独立的 PPI 技术，包括酵母双杂交系统、双分子荧光互补和串联亲和纯化，对拟南芥的核心细胞周期进行了分析。在这里，我们将这三个相互作用组与文献整理和计算预测的相互作用合并，为植物核心细胞周期机制的全面图景铺平了道路。特定于平台的相互作用揭示了每种检测方法的优缺点，并深入了解细胞周期蛋白之间相互作用的性质。此外，对获得的数据进行比较表明，只有当并行应用多种技术时，才能获得完整的相互作用组。
